OBJECTIVES The present study was aimed to investigate the α-amylase inhibition and antioxidant activities of methanolic extract of Amaranthus caudatus Linn (MeAc). METHODS Methanolic extract of Amaranthus caudatus was screened for α-amylase inhibition activity by CNPG3 method (2-chloro-p-nitrophenyl-α-D-maltotrioside) and antioxidant activity was evaluated by 1,1-diphenyl-2-picryl-hydrazile (...

Abstract Background Tetracarpidium conophorum is one of the numerous folklore medicinal plants for managing diabetes but mode action and bioactive compounds responsible antihyperglycemic property are missing in literatures. This study aimed at investigating possible modes its using both in-vitro ex-vivo methods. Powdered seed (TECOSE) was extracted with methanol standard extraction procedure. G...

This study reports the purification and biochemical characterization of thermostable and acidic-pH-stable α-amylase from Bacillus sp. Iranian S1 isolated from the desert soil (Gandom-e-Beryan in Lut desert, Iran). Amylase production was found to be growth associated. Maximum enzyme production was in exponential phase with activity 2.93 U ml-1 at 50°C and pH 5. The enzyme was purified by isoprop...

Steeping milled sorghum in up to 0.4% NaOH was investigated as a method of tannin inactivation. NaOH steeping substantially reduced assayable total phenols and tannins in both Type III and Type II sorghums and with Type III sorghum caused a 60-80% reduction in α-amylase inhibition compared to a 20% reduction by water steeping. NaOH treatment also reduced starch liquefaction time and increased f...

In the present study 4 thermophilic actinomycetes were isolated from banana waste compost and were studied for their morphological microscopical and enzyme activity. Each isolates were subjected to starch hydrolysis to select high efficient genera for αamylase production. Among the genera isolated genus Streptomyces sp. exhibited high α-amylase production. The observations showed that Streptomy...

Objective: The present study is to synthesize nanoparticles using the leaf extracts of Mimosa pudica and screen for its α-amylase inhibiting potential as well as antibacterial properties. Methods: Silver nanoparticles were synthesized using extract of Mimosa pudica leaves under various conditions such as sunlight, UV and room temperature. The synthesized nanoparticles were further characterized...

Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by Aspergillus awamori Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (Citrus sinensis var mosambi) peels as solid substrate whereas, α-amylase was produced from A. oryzae (IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohy...

OBJECTIVE To evaluate the anti-hyperglycemic potential of sinigrin using in vitro, in silico and in vivo streptozotocin (STZ) induced hyperglycemic zebrafish model. METHODS The in vitro enzyme inhibition assay was carried out to determine the IC50 value against α-glucosidase and α-amylase, in silico molecular docking was performed against both enzymes with PyRx tool and simulations were perfo...

The inhibition of enzymes involved in the breakdown of carbohydrates is considered a therapeutic approach to the management of type-2 diabetes. This study sought to investigate the effects of essential oil from clove bud on α-amylase and α-glucosidase activities. Essential oil from clove bud was extracted by hydrodistillation, dried with anhydrous Na2SO4 and characterized using gas chromatograp...