This study aims at exploring earthworm protein hydrolysate and its peptide fractions as a potential hypoglycemic agent by inhibiting α-amylase α-glucosidase. Firstly, the best hydrolysis conditions to gain hydrolysates with highest inhibitory activity (α-AIA) α-glucosidase (α-GIA) were figured out using one-factor test. Next, stability of their 5 recovered ultrafiltration membranes assessed emp...

Background: Chrysobalanus orbicularis is commonly used as food, the seeds as a spice for the popular pepper soup, and the leaves are traditionally utilized for managing type II diabetes mellitus (T2DM) in the Niger Delta area of Nigeria. Due to the limited scientific evidence on C. orbicularis, this study investigated its antioxidant and inhibitory properties against major enzymes linked to T2D...

Alpha-glucosidase inhibitors currently form an important basis for developing novel drugs for diabetes treatment. In our preliminary tests, the ethyl acetate fraction of Phlomis tuberosa extracts showed significant α-glucosidase inhibitory activity (IC₅₀ = 100 μg/mL). In the present study, a combined method using Sepbox chromatography and thin-layer chromatography (TLC) bioautography was develo...

application of chemical pesticides has increased significantly worldwide and has raised serious concerns about environmental pollutions. one of the encouraging trends to minimize pesticide risk is production of resistant plants containing toxic proteins against insect pests. considering the importance of purification and characterization of digestive enzymes in the production of resistant plant...

An in vitro antidiabetic activity on α -amylase and α -glucosidase activity of novel 10-chloro-4-(2-chlorophenyl)-12-phenyl-5,6-dihydropyrimido[4,5-a]acridin-2-amines (3a-3f) were evaluated. Structures of the synthesized molecules were studied by FT-IR, (1)H NMR, (13)C NMR, EI-MS, and single crystal X-ray structural analysis data. An in silico molecular docking was performed on synthesized mole...

The periodic rise and frequency of diabetes mellitus (DM) have pressured into the quest for other possible anti-diabetic remedies from medicinal plants. foremost strategies engaged in lessening gastrointestinal glucose levels by hindrance carbohydrate digesting enzyme prominently as α-glucosidase . Acarbose, currently used an inhibitor, is a useful treatment option management hyperglycemia type...

α-Amyrenone and β-amyrenone are triterpenoid isomers that occur naturally in very low concentrations in several oleoresins from Brazilian Amazon species of Protium (Burseraceae). This mixture can also be synthesized by oxidation of α,β-amyrins, obtained as major compounds from the same oleoresins. Using a very simple, high yield procedure, and using a readily commercially available mixture of α...

An efficient and rapid affinity-based screening method for directly fishing out natural alpha-glucosidase inhibitors from Cyperus. rotundus extract by using immobilized enzyme technology combined with UHPLC-QTOF MS analysis was established. As a results, without time-consuming and laborious isolation workload and false positive interference, five natural alpha-glucosidase inhibitors were succes...

TROJANOVÁ I., RADA V. (2005): Enzymatic activity in fermented milk products containing bifidobacteria. Czech J. Food Sci., 23: 224–229. Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus and Bifidobacterium animalis strains were tested for α-galactosidase, β-galactosidase and α-glucosidase activities. Commercially available yoghurts with bifidobacteria were also tested. Whi...

Benzothiazole-triazole derivatives 6a-6s have been synthesized and characterized by ¹HNMR and 13C-NMR. All synthetic compounds were screened for their in vitro α-glucosidase inhibitory activity by using Baker's yeast α-glucosidase enzyme. The majority of compounds exhibited a varying degree of α-glucosidase inhibitory activity with IC50 values between 20.7 and 61.1 μM when compared with standar...