1. The amino acid sequence of the amino-terminal portion of the glycine-rich, arginine-rich histone (Residues 1 to 49) has been determined from studies on the tryptic, chymotryptic, and thermolysin peptides. This sequence overlaps with that of the carboxylterminal portion (Residues 50 to 102) defined previously to give the following sequence: acetylserGly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys...

A column-switching high-performance liquid chromatography (HPLC) system for the determination of phosphopeptides has been developed. The method is based on the selective adsorption of phosphopeptides on a titania (TiO2) precolumn and successive HPLC separation of the phosphopeptides on an anion-exchange column with a UV detector (215 nm). The recoveries of phosphopeptides were tested using auth...

Freshwater extracts of starfish ovaries were used to purify the sperm-attracting peptide 'startrak' from Pycnopodia helianthoides using hydrophobic interaction chromatography and DEAE-high-pressure liquid chromatography. Partially purified attractant had a molecular mass of 13 kDa, estimated from gel filtration and polyacrylamide gel electrophoresis results. The purified attractant was subjecte...

A synthetic peptide (SP-10-IIIB) with an amino acid sequence [Cys-Thr-Arg-Pro-Asn-Asn-Asn-Thr-Arg-Lys-Ser-Ile-Arg-Ile-Gln-Arg-Gly-Pro -Pro-Gly-(Tyr); amino acids 303-321] from the human immunodeficiency virus (HIV) isolate human T-cell lymphotropic virus type III (HTLV-III) HTLV-IIIB envelope glycoprotein gp120 was coupled to tetanus toxoid and used to raise goat antibodies to HIV gp120. Goat a...

Oligopeptidases of starter and nonstarter lactic acid bacteria contribute to the proteolytic events important in maturation and flavor development processes in cheese. This paper describes the molecular cloning, expression, and specificity of the oligopeptidase PepO from the probiotic nonstarter strain Lactobacillus rhamnosus HN001 (DR20). The pepO gene encodes a protein of 70.9 kDa, whose prim...

A fast, label-free, and multiplexed method based on piezoresistive cantilevers is reported for the detection of specific protein conformations at the nanoscale level. The ligand-binding domain of the human oestrogen receptor (ERalpha-LBD) is used as the experimental model system, and ERalpha-LBD with or without oestradiol (E2) is detected using the conformation-specific peptides alpha/betaI (Se...

bradykinin in man. Circulation 29: 66, 1964 32. Oparil S, Koerner T, O'Donoghue JK: Mechanism of angiotensin I converting enzyme inhibition by SQ 20,881 (Glu-Trp-ProArg-Pro-Gln, Ile-Pro-Pro) in vivo. Further evidence for extrapulmonary conversion. Hypertension 1: 13, 1979 33. Sancho JRE, Burton R, Barger AC, Haber E: The role of the renin-angiotensin-aldosterone system in cardiovascular homeost...

A polypeptide was isolated from acid extracts of porcine hypothalami on the basis of its high ability to stimulate the release of corticotropin from superfused rat pituitary cells. After an initial separation by gel filtration on Sephadex G-25, further purification was carried out by reversed-phase HPLC. The isolated material was homogeneous chromatographically and by N-terminal sequencing. Bas...

Two peptic fragments (residues 37-88 and 43-88) of guinea pig myelin basic protein which are capable of inducing experimental allergic encephalomyelitis in Lewis rats were cleaved to shorter fragments with alpha-protease (Crotalus atrox proteinase, EC 3.4.24.1) and thermolysin (EC 3.4.24.4). The fragments were isolated, purified, and identified by amino acid composition and NH2- and COOH-termin...

Cystatin, a cysteine proteinase inhibitor, was isolated from chum salmon (Oncorilynch"s keta) pituitary glands by ion-exchange chromatography on Mono-Q, gel filtration on Superdex 75, and reverse-phase HPLC on an ODS fo1]owing ethanol-ammonium acetate extraction. Salmon pituitary cystatin was equipotent te chicken egg-white cystatin in the papain inhibitory assay. The cystatin consists of 111 a...