Human influenza is a highly contagious acute respiratory illness that is responsible for significant morbidity and excess mortality worldwide. In addition to neutralizing antibodies, there are antibodies that bind to influenza virus-infected cells and mediate lysis of the infected cells by natural killer (NK) cells (antibody-dependent cellular cytotoxicity [ADCC]) or complement (complement-depe...

An investigation of cell-mediated cytotoxicity in 22 patients with systemic lupus erythematosus (SLE), using both whole blood and purified peripheral blood mononuclear cells (PBM) to measure antibody-dependent (ADCC) and phytohaemagglutinin (PHA)-induced lymphocyte cytotoxicity for Chang liver cells, has revealed 2 distinct abnormalities in patients with active disease. PHA-induced cytotoxicity...

Background For the development of biosimilar monoclonal antibodies (mAb) or related substances containing the IgG Fc part it is mandatory to fully compare immunological properties between originator and biosimilar in a “comparability exercise” [1]. The most complex Fc associated function to mediate antibody dependent cellular cytotoxicity (ADCC) needs to be characterized using the active substa...

Previous studies have shown that sera from HIV-1-infected individuals contain antibodies able to mediate antibody-dependent cellular cytotoxicity (ADCC). These antibodies preferentially recognize envelope glycoprotein (Env) epitopes induced upon CD4 binding. Here, we show that a highly conserved tryptophan at position 69 of the gp120 inner domain is important for ADCC mediated by anti-cluster A...

One hundred and thirty nine observations of antibody-dependent cell mediated cytotoxicity (ADCC) were made on 77 with rheumatoid arthritis (RA) and 17 healthy controls. There were no differences in ADCC between these 2 groups or within the RA group with regard to disease activity, duration, seropositivity, or drug treatment. Sixty observations of phytohaemagglutinin induced cytotoxicity were ma...

The ability of neutrophils to serve as effectors of antibody-dependent cell-mediated cytotoxicity (ADCC) was studied using herpes-simplex-virus-infected (HSV-infected) human embryonic lung cells as targets. Killing of infected cells by neutrophils required HSV-immune serum and did not take place in the presence of nonimmune serum or in the absence of serum. Uninfected cells were not killed. ADC...

3F8 is a murine monoclonal IgG3 antibody specific for the tumor-associated antigen ganglioside G . Previous in vitro studies suggest that tumor regressions observed in a phase I clinical trial of 3F8 may be attributable to complement activation by 3F8 and to 3F8-dependent cellular cytotoxicity (ADCC) with lymphocytes. We now describe 3F8-mediated ADCC of G -positive tumor targets (melanoma and ...

Small-cell lung cancer (SCLC) easily recurs with a multidrug resistant phenotype. However, standard therapeutic strategies for relapsed SCLC remain unestablished. We found that human epidermal growth factor receptor 2 (HER2) is not only expressed in pretreated human SCLC specimens, but is also upregulated when HER2-positive SCLC cells acquire chemoresistance. Trastuzumab induced differential le...