This paper describes a convenient methodology for patterning substrates for cell culture that allows the positions and dimensions of attached cells to be controlled. The method uses self-assembled monolayers (SAMs) of terminally substituted alkanethiolates (R(CH2)11-15S-) adsorbed on optically transparent films of gold or silver to control the properties of the substrates. SAMs terminated in me...

Substrate-attached materials (SAMs) are cellular feet that remain on substrates after the treatment of adherent cells with EGTA. SAMs are thought to contain cell adhesion machineries, but their biochemical properties have not been addressed in detail. To gain insight into the molecular mechanisms operating in cell adhesions, we comprehensively identified the protein components of SAMs by liquid...

Calcium phosphate (CaP) ceramics are used in orthopedics and dentistry due to their excellent osseointegration and biocompatibility. The electrodeposition of CaP on titanium alloy covered with self-assembled monolayers (SAMs) was studied with respect to the influence of chain length, end-group charge, and anchoring group. SAMs with end-groups similar to the functional groups on the side chains ...

Self-assembled monolayers (SAMs) on gold derived from the direct adsorption of thioacetic acid S-decyl ester (C10SAc) and thioacetic acid S-octadecyl ester (C18SAc) were compared to the corresponding SAMs derived from the analogous adsorption n-decanethiol (C10SH) and n-octadecanethiol (C18SH). All SAMs were characterized using ellipsometry, contact angle goniometry, polarization modulation inf...

This work examines charge transport (CT) through self-assembled monolayers (SAMs) of oligoglycines having an N-terminal cysteine group that anchors the molecule to a gold substrate, and demonstrate that CT is rapid (relative to SAMs of n-alkanethiolates). Comparisons of rates of charge transport-using junctions with the structure Au(TS)/SAM//Ga2O3/EGaIn (across these SAMs of oligoglycines, and ...

This paper describes the oriented nucleation of calcite controlled by self-assembled monolayers (SAMs) of ω-terminated alkanethiols (HS(CH2)nX) supported on metal films. The effect of the chemistry and geometry of the organic surfaces was surveyed by using different functional groups (X ) CO2, SO3, PO3, OH, N(CH3)3, CH3) and different supporting metals (Au and Ag). Optical microscopy, SEM with ...

The structural and interfacial properties of self-assembled monolayers (SAMs) on gold derived from the adsorption of a series of 1,1,1-tris(mercaptomethyl)alkanes (i.e., CH3(CH2)mC[CH2SH]3, where m = 9, 11, 13, 15) were investigated. The new SAMs, which possess uniformly low densities of alkyl chains, were characterized by ellipsometry, contact angle goniometry, and polarization modulation infr...

Self-assembled monolayers (SAMs) of octanethiol and benzeneethanethiol were deposited on clean Pt(111) surfaces in ultrahigh vacuum (UHV). Highly resolved images of these SAMs produced by an in situ scanning tunneling microscope (STM) showed that both systems organize into a super-structure mosaic of domains of locally ordered, closely packed molecules. Analysis of the STM images indicated a (s...

Synapses play a critical role in establishing and maintaining neural circuits, permitting targeted information transfer throughout the brain. A large portfolio of synaptic adhesion/organizing molecules (SAMs) exists in the mammalian brain involved in synapse development and maintenance. SAMs bind protein partners, forming trans-complexes spanning the synaptic cleft or cis-complexes attached to ...