The alkaline serine protease asp, which was shown to be a virulence factor of Vibrio alginolyticus as a purified protein, was cloned from V. alginolyticus EPGS, a strain recently isolated from moribund Epinephelus coioides in an outbreak of vibriosis in a mariculture farm of Shenzhen. The asp null mutant was constructed by homologous recombination with suicide plasmid pNQ705-1. Compared with th...

Betanodavirus is the pathogen of viral nervous necrosis (VNN) disease that has caused mass mortality among many species of marine fish at larval stage. In this study, the efficacy of inactivated betanodavirus was evaluated by bath-immunization and bath-challenge of orange-spotted grouper (Epinephelus coioides) at early larval stage. Two kinds of chemicals were used for inactivation of the virus...

In Taiwan, the mushroom Antrodia camphorata (AC) is used for medicinal purposes, including cancer prevention and treatment. This study aimed to investigate effect of AC-supplemented diets on innate immunity disease resistance orange-spotted grouper Epinephelus coioides against two bacterial infections—Vibrio alginolyticus Streptococcus iniae. Orange-spotted groupers were fed at 0 (control), 1.5...