Cholate-solubilized chick kidney mitochondria that 1-hydroxylated 25-hydroxyvitamin-D3 (25-OH-D3) upon reconstitution also produced 10-oxo-19-nor-25-OH-D3, which co-eluted with 1,25-dihydroxyvitamin D3 (1,25-(OH)2-D3) on normal phase high performance liquid chromatography (HPLC) with hexane:propanol-2 (9:1), the traditional chromatographic system for isolating 1,25-(OH)2-D3. The 10-oxo derivati...

24-Keto-1,25-dihydroxyvitamin D3 has been identified as an intestinal metabolite of 1,25-dihydroxyvitamin D3 by ultraviolet absorbance, mass spectroscopy, and chemical reactivity. The metabolite was produced from 1,25-dihydroxyvitamin D3 and 1,24R,25-trihydroxyvitamin D3 in rat intestinal mucosa homogenates. 24-Keto-1,25-dihydroxyvitamin D3 is present in vivo in the plasma and small intestinal ...

Cyclin D3 is an important regulator for transition from G(1) to the S phase of the cell cycle. Cyclin D3 expression is associated with cell proliferation in lymphoid tissues, but its impact on clinical outcome in non-Hodgkin's lymphomas has not been studied. Therefore, we determined the clinical relevance of cyclin D3 expression in patients with diffuse large B-cell lymphoma. We examined the re...

Results 1) Total of 92 children, 74(80.4%) had sufficiently catch up. 2) 69(75%) were fl/fl, 15(16.3%) were fl/d3 and 8 (8.6%) were d3/d3 the SGA groups, compared with 81%, 18%, and 1%, respectively in the Korean controls (P=0.04). 3) The highest catch up rate was seen in the fl/d3 subgroup(86.6%), the lowest in the d3/d3 subgroup (62.5%). The differences were not significant. 3)d3/d3 GHR genot...

An adequate vitamin D status is essential to optimize muscle strength. However, whether vitamin D directly reduces muscle fiber atrophy or stimulates muscle fiber hypertrophy remains subject of debate. A mechanism that may affect the role of vitamin D in the regulation of muscle fiber size is the local conversion of 25(OH)D to 1,25(OH)2 D by 1α-hydroxylase. Therefore, we investigated in a murin...

background: recent studies have shown the immunomodulatory effect of vitamin d3 through down-regulation of toll-like receptor (tlr) expression in human monocytes. in this study, the effects of vitamin d treatment on tlr2 and tlr4 expression on monocytes derived from type 2 diabetes was investigated. materials and methods: to assess the influence of vitamin d3 on expression of tlr2 and tlr4 on m...

The 25-hydroxyvitamin D3 (25-OH-D3) is a nontoxic and low-affinity vitamin D receptor (VDR)-binding metabolic precursor of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]. We hypothesized that covalent attachment of a 25-OH-D3 analog to the hormone-binding pocket of VDR might convert the latter into transcriptionally active holo-form, making 25-OH-D3 biologically active. Furthermore, it might be possibl...

This study was conducted to evaluate the relative bioavailability (RBV) of 25-hydroxycholecalciferol (25-OH-D3) to cholecalciferol (vitamin D3) in 1- to 21-d-old broiler chickens fed with calcium (Ca)- and phosphorus (P)-deficient diets. On the day of hatch, 450 female Ross 308 broiler chickens were assigned to nine treatments, with five replicates of ten birds each. The basal diet contained 0....

A field experiment was conducted to examine the effects of different depths ofnitrogen (N) fertiliser placements on N accumulation, remobilisation and NO3−-Ncontent in soil of rainfed wheat. Nitrogen was applied on the surface (D1) and inthe 10 cm (D2), 20 cm (D3) and 30 cm (D4) soil layers from 2010 to 2012.Compared with D1 and D2, D3 and D4 treatments obtained significant higher Ndistribution...