مادة آلی محلول رنگی (CDOM) مقیاس مهمی در سنجش کیفیت آب است. می‌توان اذعان کرد CDOM نور موجود لایه‌های را کاهش می‌دهد و فعالیت‌های بیولوژیکی فتوسنتز مختل می‌کند سرانجام، مانع رشد جمعیت فیتوپلانکتون‌‌هایی می‌شود که برای زنجیرة غذای آبزیان ضروری‌اند. بیشتر تحقیق‌هایی تا کنون به انجام رسیده، از طول موج خاصی اندازه‌گیری ضریب جذب استفاده شده است اما، این تحقیق، امکان گسترة‌ وسیعی باندهای طیف مرئی، تع...

Post-transcriptional modifications of transfer RNAs (tRNAs) have long been recognized to play crucial roles in regulating the rate and fidelity of translation. However, the extent to which they determine global protein production remains poorly understood. Here we use quantitative proteomics to show a direct link between wobble uridine 5-methoxycarbonylmethyl (mcm5) and 5-methoxy-carbonyl-methy...

Multiple-site-specific incorporation of a noncanonical amino acid into a recombinant protein would be a very useful technique to generate multiple chemical handles for bioconjugation and multivalent binding sites for the enhanced interaction. Previously combination of a mutant yeast phenylalanyl-tRNA synthetase variant and the yeast phenylalanyl-tRNA containing the AAA anticodon was used to inc...

Maintenance of protein quality control has implications in various processes such as neurodegeneration and ageing. To investigate how environmental insults affect this process, we analysed the proteome of yeast continuously exposed to mild heat stress. In agreement with previous transcriptomics studies, amongst the most marked changes, we found up-regulation of cytoprotective factors; a shift f...

Although tRNA modifications have been well catalogued, the precise functions of many modifications and their roles in mediating gene expression are still being elucidated. Whereas tRNA modifications were long assumed to be constitutive, it is now apparent that the modification status of tRNAs changes in response to different environmental conditions. The URM1 pathway is required for thiolation ...