Polypeptides synthesized in vitro in rabbit reticulocyte lysates, directed by poly(A)+ RNA isolated from turnip leaves infected with cauliflower mosaic virus (CaMV), were analysed by polyacrylamide gel electrophoresis. Following translation of virus-specific RNA purified by hybrid-selection using CaMV DNA immobilized on DBM papers, the polypeptides observed included the viral gene VI inclusion ...

UNLABELLED Palm oil obtained from E. guineensis Jacq. Tenera is known to have about 44% of palmitic acid (C16:0). Palmitoyl-Acyl Carrier Protein Thioesterase (PATE) is one of the key enzymes involved in plastidial fatty acid biosynthesis; and it determines the level of the C16:0 assimilation in oilseeds. This enzyme's activity in oil palm is responsible for high (> 44 % in E. guineensis Jacq. T...

Cauliflower mosaic virus (CaMV) TAV protein (TransActivator/Viroplasmin) plays a pivotal role during the infection cycle since it activates translation reinitiation of viral polycistronic RNAs and suppresses RNA silencing. It is also the major component of cytoplasmic electron-dense inclusion bodies (EDIBs) called viroplasms that are particularly evident in cells infected by the virulent CaMV C...

LIGATION AND RECOMBINATION OF THE DNA OF CAULIFLOWER MOSAIC VIRUS (CAMV) IS DEMONSTRATED BY THE FOLLOWING EXPERIMENTS: (i) Ligation: Different noninfectious fragments of the CaMV genome (obtained after insertion into plasmid pBR322 followed by enzymatic excision) regained infectivity when mixtures of them were used to inoculate their host. The symptom appearance was delayed by comparison with a...

Natural mixed infections of plant viruses are frequent, often leading to unpredictable variations in symptoms, infectivity, accumulation and/or vector transmissibility. Cauliflower mosaic caulimovirus (CaMV) has often been found in mixed infections with turnip mosaic potyvirus (TuMV) in plants of the genus Brassica. This study addressed the effect of mixed infection on infectivity, pathogenicit...

Mutagenesis analysis of the polyadenylation [poly(A)] signal from the cauliflower mosaic virus (CaMV), a plant pararetrovirus, revealed striking differences to known vertebrate poly(A) signals. Our results show that (1) the AATAAA sequence is necessary for efficient cleavage at the poly(A) site, although the requirement for an authentic AATAAA might be less stringent in plant than in vertebrate...

Rice is a major cereal crop providing food and energy to more than half of world’s population drought challenging abiotic stress limiting rice production. Engineering tolerance trait bottle neck because multigenic control complex nature. Two promising candidate genes utilized in engineering include DREB2A transcription factor (a master regulator downstream inducible genes) APX (an important ROS...

Allotopic expression is the term given for deliberate relocation of gene function from an organellar genome to nuclear genome. We hypothesized that allotopic essential mitochondrial using a promoter expressed efficiently in all cell types except those responsible male reproduction would yield cytoplasmic sterility (CMS) phenotype once endogenous was inactivated via editing. To test this, we rep...