The increase in the incidence of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella species has become a serious problem worldwide, because of their incrimination in antibiotic resistance. The objective of this study is to investigate the resistance genes responsible for ESBL-producing Klebsiella species and carbapenemase-producing Klebsiella (CRE) isolated in Mthatha and to study thei...

conclusions it was the first report on the blactx-m-15 gene emergence in clinical enterobacter spp. in iran. the current study demonstrated the predominant presence of the gene encoding ctx-m-15 among esbl producing enterobacter spp. commonly with a large plasmid, in the setting. background enterobacter spp. is increasingly recognized as an important nosocomial pathogen and implicated in many e...

This study investigated the resistance of various Salmonella strains to beta-lactam antibiotics. Salmonella Minnesota (36 strains) and Salmonella Heidelberg (24 strains) were isolated from broiler chickens and carcasses by the Disk Diffusion Test and resistance genes blaCTX-M-8, blaACC-1 and blaCMY-2 were detected by PCR. Of the 60 strains tested, 80% were resistant to at least one antibiotic. ...

The aim of this study was to explore the characteristics blaCTX-M-27 carriage and mobilization in Salmonella Escherichia coli isolates from food-producing animals China. A total 2280 E. 229 collected food June 2003 September 2014 were screened for presence gene. blaCTX-M-27-positive typed plasmid DNA sequenced determine genetic context types present. Bacterial fitness evaluated by growth curve ...

results of the 100 klebsiella isolates, 41 (41%) demonstrated resistance or reduced susceptibility to ceftazidime and/or aztreonam and 35% (n = 35) were esbl-producers. twenty-eight (8o%) of the esbl-producing isolates carried the blactx-m type genes. based on pcr assays and sequencing of blactx-m genes, ctx-m-1, ctx-m-2 and ctx-m-9 were identified in 21 (60%), 15 (42%) and nine (34%) of these ...

BACKGROUND Extended-spectrum β-lactamases (ESBLs) are enzymes capable of hydrolyzing oxyimino-β-lactams and inducing resistance to third generation cephalosporins. The genes encoding ESBLs are widespread and generally located on highly transmissible resistance plasmids. We aimed to investigate the complement of ESBL genes in E. coli and Klebsiella pneumoniae causing nosocomial infections in hos...

The aim of this work was to investigate the epidemiological and genetic characteristics of ESBL-producing Klebsiella pneumoniae (ESBL-Kp) causing community-onset infections. K. pneumoniae isolates were collected from 31 Chinese secondary hospitals between August 2010 and 2011. Genes encoding ESBL and AmpC beta-lactamases were detected by PCR. The isolates were assigned to sequence types (STs) u...

ESBL (extended-spectrum-β-lactamase)-positive Enterobacteriaceae, which colonized European soldiers in tropical Western African Mali, were subjected to a molecular assessment of their resistance determinants. By doing so, a better insight into the locally endemic pattern of ESBL-associated β-lactamase genes was aspired. From a previous study on diarrhea in European soldiers on deployment in tro...

Background Antimicrobial resistance is an important factor threatening human health. It is widely accepted that antibiotic resistant bacteria such as Escherichia coli (E. coli) released from humans and animals into the water sources, can introduce their resistance genes into the natural bacterial community. Objective The aim of this study was to investigate the prevalence of blaTEM, blaCTX, b...

OBJECTIVES Extended-spectrum beta-lactamase (ESBL) producing bacteria have an important role in nosocomial infections. Due to the limited availability of information about the molecular epidemiology of ESBL producing bacteria in Mashhad, we decided to investigate about TEM, CTX and SHV ESBLs among urinary Escherichia coli isolates in Mashhad, a city in northeast Iran. MATERIALS AND METHODS On...