A transplantable melanoma of Golden hamsters has been carried by the chunk method from the 15th through the 52nd generation and examined for growth characteristics and for light and electron microscopic appearance as well as for light microscopy histochemistry. Grossly, tumors were heavily pigmented and inva sive, and grew rapidly to kill the animals in 4 to 6 weeks. The growth rate remained st...

Nowadays we all can look around with satisfaction. " We " – the urological community, of course. Developments in surgical equipment and successes of new operational techniques contribute to the fact that urology sets the trends in contemporary medicine. Endoscopic and laparoscopic techniques seized a significant volume of interventional procedures, adding a new dimension to urology. For its sig...

For 21 strains of Salmonella enterica, nucleotide sequences were obtained for three invasion genes, spaO, spaP, and spaQ, of the chromosomal inv/spa complex, the products of which form a protein export system required for entry of the bacteria into nonphagocytic host cells. These genes are present in all eight subspecies of the salmonellae, and homologues occur in a variety of other bacteria, i...

Salmonella enterica serotype Paratyphi B is a globally distributed human-specific pathogen causing paratyphoid fever. The aim of this study was to develop a rapid and reliable polymerase chain reaction (PCR) assay for its detection in food. The SPAB_01124 gene was found to be unique to S. Paratyphi B using comparative genomics. Primers for fragments of the SPAB_01124 gene and the Salmonella-spe...

The study established a multiplex real-time polymerase chain reaction (PCR) for simultaneous detection and quantitation of Staphylococcus aureus, Salmonella Enterica, Bacillus cereus and Vibrio parahaemolyticus in the same reaction at one time, thus reducing the amounts of reagents and cutting down on the labor and time. Four sets of genetic marker specific primers corresponding to SAOUHSC_0229...

Newly developed light-up probes offer an attractive tool for PCR product detection. The light-up probe, which consists of a thiazole orange derivative linked to a peptide nucleic acid oligomer, hybridizes specifically to complementary nucleic acids. Upon hybridization the thiazole orange moiety interacts with the nucleic acid bases and the probe becomes brightly fluorescent. This eliminates the...

Salmonellosis is an important zoonotic disease with worldwide distribution. Reptile-associated Salmonellosis in humans is an increasing public health concern. This study was conducted to determine the prevalence and antimicrobial susceptibility of Salmonella isolated from snakes. Cloacal swab samples were used for isolation by conventional culture, biochemical and serological test. Confirmation...

Normal human placental metabolism has been studied in vivo by image localised 31P magnetic resonance spectroscopy in 13 women with anterior placentas; five, however, were too fat for useful spectral signals to be obtained. Magnetic resonance spectra of good quality which were considered to have arisen from the placenta were obtained from seven women with uncomplicated pregnancies (median gestat...