Aim: To examine the ciprofloxacin susceptibility of 106 Pseudomonas aeruginosa eye isolates from the United Kingdom, Denmark, India, the United States, and Australia, and to determine the molecular mechanisms of resistance. Methods: Ciprofloxacin susceptibility was tested by an agar dilution method; genomic DNA corresponding to the quinolone target genes gyrA and parC, and the regulatory genes ...

OBJECTIVES Quinolone resistance in the opportunistic pathogen Pseudomonas aeruginosa is commonly caused by mutations that alter the target molecules DNA gyrase/topoisomerase IV, or cause activation of various efflux systems. We have analysed the effect of quinolone resistance caused by DNA gyrase/topoisomerase IV mutations on bacterial fitness. METHODS Norfloxacin-resistant mutants were isola...

AIM To examine the ciprofloxacin susceptibility of 106 Pseudomonas aeruginosa eye isolates from the United Kingdom, Denmark, India, the United States, and Australia, and to determine the molecular mechanisms of resistance. METHODS Ciprofloxacin susceptibility was tested by an agar dilution method; genomic DNA corresponding to the quinolone target genes gyrA and parC, and the regulatory genes ...

30 The need for new antibiotics that address serious Gram-negative infections is well recognized. 31 Our effort in a series of novel bacterial type II topoisomerase inhibitors (designated as NBTIs) led 32 to the discovery of NBTI 5463, an agent with improved Gram-negative activity over other 33 NBTIs, in particular against Pseudomonas aeruginosa (F. Reck et al., submitted for publication). 34 I...