Compared the Effect of Red and Infrared Low-Level Lasers on Folliculogenesis Cycle with the Effect of Clomiphene Drug in Rat’s Ovarian Tissue

Introduction: Folliculogenesis is a cycle that any disruption in this cycle leads to disease. Treatment include Surgery, drugs and low-power lasers have been used as complementary. This study investigated photo-bio-stimulation effect of lasers (power<250 mW)) on folliculogenesis cycle in rats’ ovarian tissue in two wavelength 630 and 810 nm autonomously. Methods: Twenty-eight female Wistar rats in weights between 150-250g at the age of 9 weeks divided into four groups: control (CT), Drug(D), Red Laser (RL) and Near Infrared Laser (NIRL). For this, two diode lasers with an output 33±2 mw/cm2 for RL and 212±2 mw/cm2 for NIRL have been used. In the first 2 days of estrous cycle the laser groups were irradiated (5j/cm2 dose). The rats in D group received clomiphene (1μg/Kg). After the experiments, the rats were anesthetized and sacrificed and their ovarian tissues were removed. Results: The number of different follicles and the levels of effective hormones were evaluated. The results showed a change in follicles and hormones compared with D and CT groups. This change was significant in the NIRL group compared with other groups, while it was not significant in the RL group. Theas results in D group was less than laser groups and was significant only compared with CT group. Conclusions: Low-power laser therapy at 810 nm can increase the activity of ovarian tissue in the production of follicles independently. Therefore, this study was able to introduce 810 nm laser as a new independent treatment for ovarian hypoglycemia that can be used in future studies.