We examined cell isolation, viability, and growth in adipose-derived stem cells harvested from whole adipose tissue subject to different cryopreservation lengths (2-1159 days) from patients of varying ages (26-62 years). Subcutaneous abdominal adipose tissue was excised during abdominoplasties and was cryopreserved. The viability and number of adipose-derived stem cells isolated were measured a...

Bone defect is one of the challenges for dentists in process healing bone tissue. can occur alveolar with etiology microorganisms and cyst expansion. In addition, cases defects are also often found treatment apex resection hemisection. Autologous graft a clinical gold standard defect. However, use has limited number growth factors produced. Tissue engineering latest method terms regeneration. t...

Junctional adhesion molecules (JAMs) are expressed in diverse types of stem and progenitor cells, but their physiological significance has yet to be established. Here, we report that JAMs exhibit a novel mode interaction biological activity adipose-derived stromal/stem cells (ADSCs). Among the JAM family members, JAM-B JAM-C were concentrated along cell membranes mouse ADSCs. not was broadly di...

the effects of mobile phone exposure on humans, due to its potential health hazards, have become the focus of interest since many years ago. the effect of global system for mobile communications (gsm 900 mhz) on growth and proliferation rate of mesenchymal stem cells was assessed at the different distances and intensities of radiation. a subscriber identity module (sim) card was inserted to thi...

This study compares mesenchymal cells isolated from excised burn wound eschar with adipose-derived stem cells (ASCs) and dermal fibroblasts in their ability to conform to the requirements for multipotent mesenchymal stem cells (MSCs). A population of multipotent stem cells in burn eschar could be an interesting resource for tissue engineering approaches to heal burn wounds. Cells from burn esc...

The mechanical properties of single cells play important roles in regulating cell-matrix interactions, potentially influencing the process of mechanotransduction. Recent studies also suggest that cellular mechanical properties may provide novel biological markers, or "biomarkers," of cell phenotype, reflecting specific changes that occur with disease, differentiation, or cellular transformation...

In cell therapy and regenerative medicine, a reliable source of stem cells together with cytokine growth factors and biomaterial scaffolds seem necessary. As adipose tissue is easy accessible and is abundant source of adult stem cells and can differentiate along multiple lineages, it can be considered as a good candidate in aesthetic medicine. The clinical application of adipose-derived stem ce...

Background Cleft palate (CP) is a common congenital defect. It makes serious difficulties for cleft-affected children. The gold standard of care is autogenous bone grafting which may cause additional problems in donor site along with disappointing results. Tissue engineering is a promising solution for a widespread range of defects and disorders. It is reasonable to utilize this novel technolo...

OBJECTIVES Understanding the changes in chondrogenic gene expression that are involved in the differentiation of human adipose-derived stem cells to chondrogenic cells is important prior to using this approach for cartilage repair. The aims of the study were to characterize human adipose-derived stem cells and to examine chondrogenic gene expression after one, two, and three weeks of induction....

BACKGROUND Stem cell-enriched fat grafting has been proposed as a potential therapy for reconstructive, restorative, or enhancement-related procedures of the breast. Its role in postoncologic breast reconstruction is still emerging, with concerns about safety. The authors investigated the dose-dependent interaction between human adipose-derived mesenchymal stromal cells (AD-MSCs) and human brea...