Sexual reproduction is a critical process in the life-cycle of plants and very sensitive to environmental perturbations. To better understand the effect of high temperature on plant reproduction, we cultivated tomato (Solanum lycopersicum) plants in continuous mild heat. Under this condition we observed a simultaneous reduction in pollen viability and appearance of anthers with pistil-like stru...

The objective of this study is to investigate the occurrence poor fruit set in Telfairia occidentalis , despite prolific production flowers plant. Consequently, floral morphology male and female plants with respect to: pollen viability, size count distribution for aneuploids polyploids were examined. Floral buds from different ( occidentalis) collected viability was assessed using aceto-carmine...

We present the first records on pollination biology of Jacquemontia montana (Moric.) Meisn. (Convolvulaceae), a widespread climber in the Chapada Diamantina. Our objectives were to (1) characterise flower morphology and biology of J. montana; (2) sample flower visitors and (3) make inferences about potential pollinators, based on foraging behaviour. Observations and sampling were performed on t...

Pollen undergo a maturation process to sustain pollen viability and prepare them for germination. Molecular mechanisms controlling these processes remain largely unknown. Here, we report an Arabidopsis thaliana mutant, dayu (dau), which impairs pollen maturation and in vivo germination. Molecular analysis indicated that DAU encodes the peroxisomal membrane protein ABERRANT PEROXISOME MORPHOLOGY...

The entire pollen life span is driven by polyamine (PA) homeostasis, achieved through fine regulation of their biosynthesis, oxidation, conjugation, compartmentalization, uptake, and release. The critical role of PAs, from microsporogenesis to pollen-pistil interaction during fertilization, is suggested by high and dynamic transcript levels of PA biosynthetic genes, as well as by the activities...

Pollen exine is essential for protection from the environment of the male gametes of seed-producing plants, but its assembly and composition remain poorly understood. We previously characterized Arabidopsis (Arabidopsis thaliana) mutants with abnormal pollen exine structure and morphology that we named kaonashi (kns). Here we describe the identification of the causal gene of kns4 that was found...

Rapid Alkalinization Factors (RALFs) are plant peptides that rapidly increase the pH of plant suspension cell culture medium and inhibit root growth. A pollen-specific tomato (Solanum lycopersicum) RALF (SlPRALF) has been identified. The SlPRALF gene encodes a preproprotein that appears to be processed and released from the pollen tube as an active peptide. A synthetic SlPRALF peptide based on ...

The evaluation of pollen viability and fruit setting capacity are two essential criteria for pollinator’s characterization. This study was carried out to evaluate Fertility/ in five Varieties Tomatoes. We analyzed the grains different varieties tomatoes grown ITM University, Gwalior, Madhya Pradesh, India. Aceto- carmine method is used estimate viability. germination percentage, Fruit rate were...

Plant genetic transformation has arguably been the core of plant improvement in recent decades. Efforts have been made to develop in planta transformation systems due to the limitations present in the tissue-culture-based methods. Herein, we report an improved in planta transformation system, and provide the evidence of reporter gene expression in pollen tube, embryos and stable transgenicity o...

Cold stress modifies anthers' metabolic pathways to induce pollen sterility. Cold-tolerant plants, unlike the susceptible ones, produce high proportion of viable pollen. Anthers in susceptible plants, when exposed to cold stress, increase abscisic acid (ABA) metabolism and reduce ABA catabolism. Increased ABA negatively regulates expression of tapetum cell wall bound invertase and monosaccharid...