BACKGROUND Although fungal phytase is frequently used as an additive to animal feed few investigations of its allergenic property have been conducted. METHODS Fifty-three subjects occupationally exposed to powdered phytase from Aspergillus niger were studied. Exposure data and symptoms were registered by the company physician. RESULTS Thirty-eight subjects complained of work-related respira...

Phytases are phosphohydrolytic enzymes that initiate stepwise removal of phosphate from phytate. Simple-stomached species such as swine, poultry, and fish require extrinsic phytase to digest phytate, the major form of phosphorus in plant-based feeds. Consequently, this enzyme is supplemented in these species' diets to decrease their phosphorus excretion, and it has emerged as one of the most ef...

Two hundred sixteen previously molted Dekalb Delta Leghorn laying hens (78 wk of age) were utilized to determine the effect of phytase on the ileal digestibility of amino acids. The hens were randomly arranged in a 3 x 2 factorial to evaluate three diet types that were corn-soybean meal (C-SBM), C-SBM-meat and bone meal (C-SBM-MBM), and C-SBM-wheat middlings (C-SBM-WM) and two levels of phytase...

The effect of a thermotolerant 6-phytase produced by Trichoderma reesei on performance and apparent total tract digestibility (ATTD) of P and Ca was evaluated in 192 weaned piglets (randomized block design; 16 replicates; 2 piglets each). Diets based on wheat (Triticum aestivum), barley (Hordeum vulgare), oat (Avena sativa), soybean (Glycine max) meal, and whey protein with adequate [positive c...

Phytases are nutritionally important for increased bioavailability of dietary minerals and phosphate for monogastric animals including humans. Release of minerals and phosphate is accomplished by the enzymatic stepwise degradation of phytate (inositol hexaphosphate, IP6). Activity determinations of phytase is often based on analysis of total released phosphate (Pi), but phytase activity in its ...

The effect of the ingestion of myo-inositol hexaphosphate (IP6) and phytase (EC 3.1.3.26) on the digestibility of casein was investigated using growing broiler chickens. A total of 64 female Ross broilers were used in a precision feeding study. One group of 8 birds was fed a solution of glucose to estimate endogenous losses. Seven groups, each of 8 birds, were fed either casein, casein + 1,000 ...

An experiment was conducted to determine the effect of dietary phytic acid (PA) and phytase supplementation on small intestinal histomorphology and Na-dependent glucose transporter 1 (SGLT1) gene expression in piglets. Twenty-four piglets with an average initial BW of 7.60 ± 0.73 kg were randomly assigned to 3 experimental diets, to give 8 piglets per diet. The diets were a casein-cornstarch-ba...

Eighteen gilts (initial BW of 10.9 kg; age 4 wk) were used in a 35-d experiment to study the effects of adding microbial phytase to a pearl millet-soybean meal-based diet on growth, apparent digestibility and retention of nutrients, and bone mineral status. The dietary treatments were arranged in a 2 x 3 factorial with two levels of P (.58%, low-P or .95%, adequate-P) and three levels of microb...

Phytase can improve the nutritional value of plant-based foods by enhancing protein digestibility and mineral availability through phytate digestion in the stomach and the food processing industry. Microbial sources are more promising for the production of phytases on a commercial scale. The objectives of this exploration were to screening and isolation of phytase-producing bacteria from hot sp...

In the present study phytase, phytate and protein content were determined in twenty wheat varieties. Among twenty wheat varieties, LOK-1 variety was found to have higher content of phytase, phytate and protein. During the study, phytase activity was 197.30±0.65 to 2132.77±0.29 nM/min/g, phytate 2.90±0.35 to 7.07±0.09 and protein 77.07±0.64 to 148.40±0.31 mg/g found, respectively through differe...