The synthetic oligodeoxyribonucleotide pCGAAAGACTACAC has been applied as a site-specific mutagen to introduce a T leads to G transversion mutation at nucleotide position 1223 of the M13 DNA sequence. The in vitro-induced conversion of a TAT codon into a TAG at this position resulted in gene IX mutants with an amber mutant character thereby confirming that this reading frame defines a gene of a...

Web extraction is the task of turning unstructured HTML into structured data. Previous approaches rely exclusively on detecting repeated structures in result pages. These approaches trade intensive user interaction for precision. In this paper, we introduce the Amber (“Adaptable Model-based Extraction of Result Pages”) system that replaces the human interaction with a domain ontology applicable...

Amber is of great paleontological importance because it preserves a diverse array of organisms and associated remains from different habitats in and close to the amber-producing forests. Therefore, the discovery of amber inclusions is important not only for tracing the evolutionary history of lineages with otherwise poor fossil records, but also for elucidating the composition, diversity, and e...

We describe the generation of a complete set of orthogonal 21st synthetase-amber, ochre and opal suppressor tRNA pairs including the first report of a 21st synthetase-ochre suppressor tRNA pair. We show that amber, ochre and opal suppressor tRNAs, derived from Escherichia coli glutamine tRNA, suppress UAG, UAA and UGA termination codons, respectively, in a reporter mRNA in mammalian cells. Acti...

OBJECTIVE To verify the hydrogen-ion potential (pH) of dobutamine hydrochloride solutions under environmental conditions similar to those of neonatal intensive care units. METHODS We analyzed the pH of the drug diluted in 5% dextrose in water or 0.9% NaCl under different conditions of temperature (22 and 37 degrees C) and light (dark, fluorescent light bulbs, and phototherapy equipment), usin...

AMBER is a model of first language acquisition that improves its performance through a process of error recovery. The model is implemented as an adaptive production system that introduces new condition-action rules on the basis of experience. AMBER starts with the ability to say only one word at a time, but adds rules for ordering goals and producing grammatical morphemes, based on comparisons ...