background: visceral leishmaniasis (vl) as one of the most important human parasitic disease is endemic in some parts of iran. several cases of vl have been reported recently in the ilam province. the current study aimed to assess the pre-sent status of human vl in the region. methods: a random cluster sampling method was used to collect 456 serums samples from the children up to 12 years of ag...

in this study, the effects of different levels of garlic on performance and humoral immunity of broiler chicks were investigated. three hundred eighty four broiler chicks (arian) from day one were divided into two groups of male and female. animals received four treatments diet including: 0.0% (control), 0.1% garlic powder, 0.1% garlic tablet and 0.5% garlic tablet from day 3 to 42 of age. a fa...

The rate of agglutination by polylysine of young and old human erythrocytes was automatically recorded and correlated with measurements of the electrophoretic mobility of these cells. Old red cells have a reduced electrophoretic mobility as compared with young cells, as well as a higher rate of agglutination than young cells. Mild treatment of red cells with receptor destroying enzyme (RDE) whi...

A modified rapid slide agglutination test for the presumptive identification of Brucella canis infection in dogs has been developed. The method required mixing 0.1 ml of canine serum with 0.1 ml of 0.2 M 2-mercaptoethanol solution. Equal volumes (0.05 ml) of the treated serum and the B. canis plate antigen were mixed. Agglutination results were read within 2 min. Clinical studies showed 100% ag...

Ruthenium red induces the agglutination of ascites hepatoma cells. The agglutination was inhibited by acidic polysaccharides but not by haptenic inhibitors of some plant agglutinins. The agglutination was also abolished by treatment of the cells with a low concentration of papain. Papain digestion causes a decrease in the number of microvilli on the cell surface and the release of carbohydrate-...

A comparison is made of tests for thyroglobulin antibody, using gel diffusion, electroprecipitin, bentonite flocculation, tanned red cell agglutination, and latex slide agglutination techniques on sera from cases of Hashimoto's disease and other thyroid disorders. Any increase in gamma globulin was also noted from the serum electrophoretic pattern. The gel diffusion and electroprecipitin tests ...

background: a β-mercaptoethnol (β-me)-treated promastigote antigen of l. donovani was successfully employed in direct agglutination test (dat) for the diagnosis of visceral leishmaniasis (vl). objective: the β-me-treated antigen was further incorporated into an enzyme-linked immunosorbent assay set-up (β-me elisa) and evaluated for vl diagnosis against outcome of reference freeze-dried dat (fd-...

A flow-induced cell movement assay combined with a surface plasmon resonance (SPR) technique was developed to quantify the agglutination strength, derived from the standard tube-agglutination test. Red blood cells (RBCs), based on the ABO blood group system, were specifically captured by anti-A and/or anti-B antibodies immobilized on a sensor surface. The agglutination strength corresponds to t...

background: toxoplasma gondii infection is widely prevalent in many species of warm-blooded animals including human. the aim of this study was to determine the prevalence of antibodies to t. gondii from slaughtered sleep and goats by mod­ified agglutination test (mat) in kerman region , southeastern iran. methods : altogether 1340 blood samples were collected from 562 sheep and 778 goats from a...

Non-specific reactions in agglutination tests have long provided trouble for the bacteriologist. One of the chief offenders has been spontaneous agglutination. The pseudo-reactions obtained in the serologic agglutination of the obligate anaerobes Clostridium paraputrificum (1934) and Clostridium capitovalis (1935), however, were not considered as due to spontaneous agglutination, but rather to ...