Objective- To evaluate the biological compatibility of differentiated stem cells embedded in poly-vinyl-alcohol (PVA) scaffolds for repair of distal femoral cartilage defect.   Design- Experimental in vivo study. Animals- Twelve adult male New Zealand white rabbits were used which were divided into two groups (I, II) six rabbits each.   Procedures- Mesenchymal stem cells were isolated from h...

background cartilage tissue has limited regenerative capacity and the management of cartilage defects has always been a challenging issue. platelet-rich plasma (prp) has been recently been used to improve healing of cartilage defects. in the present experiment, we aimed to investigate the effects of prp on regeneration capacity as well as survival of the cartilage grafts in a rabbit model. meth...

Recently, it has been proven that cartilage healing is difficult. The most commonly used treatments are autogenously cartilage grafting and allogeneic bone grafting, but grafts cannot fully meet treatment goals because of source, price, safety, and other concerns. Thus, a combination of biological materials and tissue engineering technology has become a recent trend in studies. Among the studie...

The importance of mesenchymal stem cells (MSCs), as adult stem cells (ASCs) able to divide into a variety of different cells is of utmost importance for stem cell researches. In this study, the ability of cartilage extract to induce differentiation of rat derived omentum tissue MSCs (rOT-MSCs) into chondrocyte cells (CCs) was investigated. After isolation of rOT-MSCs, they were co-cultured with...

Given the difficulties in accessing articular cartilage as a source of chondrocytes to be used in fabricating cartilage constructs, alternative sources are required. The present study examined chondrocytes from costal cartilage for their suitability in cartilage tissue engineering. Chondrocytes isolated from rat knee and rib hyaline cartilage were separately mixed with alginate and placed in a ...

Background: Previous studies have shown that Galectin-3, a member of lectin family, is expressed in developing cartilage in mouse embryo and also in growth plate of long bones.   Objective: In the present work, the expression pattern of Galectin-3 in normal and various grades of osteoarthritic (OA) human articular cartilage has been studied.   Methods: Using immunohistochemistry and standard we...

background dedifferentiation of chondrocytes remains a major problem for cartilage tissue engineering. chondrocytes loss differentiated phenotype in in vitro culture that is undesired for repair strategies. the chondrocyte is surrounded by a pericellular matrix (pcm), together forming the chondron. pcm has a positive effect on the maintenance of chondrocyte phenotype during culture in compariso...

Background: Hyaline cartilage defects exhibit a major challenge in the field of orthopedic surgery owing to its limited repair capacity. On the other hand, mesenchymal stem cells (MSCs) are regarded as potent cells with a property of cartilage regeneration. We aimed to optimize marrow-derived MSC chondrogenic culture using a small bioactive molecule referred to as BIO. Methods: MSCs from the ma...

Background: Integrins are a family of transmembrane proteins that allow communication between the extracellular matrix and the interior of cells. Chondrocytes, cells of articular cartilage, express integrins and these molecules appear to have a variety of roles including mechanotransduction. Integrins are known to associate with a number of accessory molecules such as CD147 that may act to regu...

objective(s) articular cartilage tissue defects cannot be repaired by the proliferation of resident chondrocytes. autologous chondrocyte transplantation (act) is a relatively new therapeutic approach to cover full thickness articular cartilage defects by in vitro grown chondrocytes from the joint of a patient. therefore, we investigated the redifferentiation capability of human chondrocytes mai...