Human induced pluripotent stem cells (hiPSC) have been shown to differentiate into a variety of replacement cell types. Detailed evaluation and comparison with their human embryonic stem cell (hESC) counterparts is critical for assessment of their therapeutic potential. Using established methods, we demonstrate here that hiPSCs are capable of generating hemangioblasts/blast cells (BCs), endothe...

Recently, significant progress has been made in ART for the treatment of male infertility. However, current ART has failed to help infertile patients with non-obstructive azoospermia, unless donor sperm is used. In fact, most couples wish to have their own genetically related child. Human induced pluripotent stem cells (hiPSCs) can be generated from patients' somatic cells and in vitro derivati...

OBJECTIVE There is constant difficulty in obtaining adequate supplies of blood components, as well as disappointing performance of "universal" red blood cells. Advances in somatic cell reprogramming of human-induced pluripotent stem cells (hiPSCs) have provided a valuable alternative source to differentiate into any desired cell type as a therapeutic promise to cure many human disease. MATERI...

Innovative applications of cell therapy products (CTPs) derived from human pluripotent stem cells (hPSCs) in regenerative medicine are currently being developed. The presence of residual undifferentiated hPSCs in CTPs is a quality concern associated with tumorigencity. However, no simple in vitro method for direct detection of undifferentiated hPSCs that contaminate CTPs has been developed. Her...

A large amount of research has been devoted to developing skin organs for studying developmental pathways, modeling diseases, or regenerative medicine purposes. However, the field is still far from fulfilling its dream creating appendages, such as hair follicles and sweat glands. This challenge could be addressed with use human induced pluripotent stem cells (hiPSCs). Nevertheless, current hiPS...

Human ES cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are usually generated and maintained on living feeder cells like mouse embryonic fibroblasts or on a cell-free substrate like Matrigel. For clinical applications, a quality-controlled, xenobiotic-free culture system is required to minimize risks from contaminating animal-derived pathogens and immunogens. We previously repo...

Human induced pluripotent stem cells (hiPSCs) are a promising cellular source in cell therapies or regenerative medicine and they allow to address fundamental mechanisms embryo-genesis. However, their culture provide large amounts of high quality remains challenging. Here, using an encapsulation microfluidic device, we observe that hiPSCs self-organize into cysts, which spherical closed epithel...

Human induced pluripotent stem cell (hiPSC)-derived neural cells provide valuable disease models for pathophysiological analysis and drug discovery intractable neurodegenerative diseases. However, differentiation of hiPSCs requires a complex long culture procedure, which has been bottleneck analysis. We previously demonstrated rapid, efficient, simple motor neuron from human (hPSCs). Although o...

The regulation of transcription and genome stability by epigenetic systems are crucial for the proper development of mammalian embryos. Chemicals that disturb epigenetic systems are termed epimutagens. We previously performed chemical screening that focused on heterochromatin formation and DNA methylation status in mouse embryonic stem cells and identified five epimutagens: diethyl phosphate (D...