introduction: the suspected water samples contaminated with legionella were enriched with acanthamoebae. small numbers of bacteria (not detectable by culture) can be multiplied in amoebae up to culturable level in co-culture condition. the conventional culture method for legionnaires' disease bacterium always suffers from low sensitivity. this co-culture method can significantly improve culture...

The importance of morphologic alteration, clumping, and subsequent fusion of blood platelets in hemostasis and thrombosis is well known. The mechanisms underlying these platelet changes, often referred to as agglutination and viscous metamorphosis, are poorly understood. Since the early work of Wright and Minot (1), many studies have suggested that thrombin may be involved. Widely divergent con...

The importance of morphologic alteration, clumping, and subsequent fusion of blood platelets in hemostasis and thrombosis is well known. The mechanisms underlying these platelet changes, often referred to as agglutination and viscous metamorphosis, are poorly understood. Since the early work of Wright and Minot (1), many studies have suggested that thrombin may be involved. Widely divergent con...

We comment on the problems faced by Niscola et al1 during a specific diagnosis of leishmaniasis in patients with hematological malignancies. Rather than a serological test like direct agglutination and anti-K39 antibody, an in-vitro culture, and/or polymerase chain reaction (PCR) might be required to establish leishmaniasis diagnosis in those with a concurrent HIV infection. Serology would gene...

Since "incomplete" or "blocking" antibodies were first demonstrated in 1944 (1, 2), a variety of techniques has been developed to demonstrate the combination of incomplete antibodies with the appropriate erythrocytes (3). These procedures utilize high protein media, enzyme-treated cells, the antiglobulin (Coombs') reaction, hypotonic environments, or isotopic labeling. Previous explanations for...

AIM To examine in vitro the effects of brief contact with various infusion solutions on red blood cells from newborn infants, as occurs in the "waste" syringe during routine blood sampling from umbilical artery catheters. The mixture of blood and solution in the "waste" syringe is usually reinfused into the baby. Reinfused red blood cells may be damaged by the infusion solution. It is hypothesi...

Morton, Harry E. (University of Pennsylvania, Philadelphia). Mycoplasma-latex agglutination reaction. J. Bacteriol. 92:1196-1205. 1966.-The building up of Mycoplasma cell mass through adsorption to carrier particles as a method for enhancing the agglutination reaction to identify Mycoplasma is described. Mycoplasma cells of human, avian, swine, goat, sewage, and tissue-culture origin were adsor...

INTRODUCTION Visceral leishmaniasis is the most severe clinical form of leishmaniasis and is often fatal without proper treatment. Therefore, early and accurate diagnosis is important, but often difficult in endemic areas. OBJECTIVE The aim was to evaluate a direct agglutination test as a potential visceral leishmaniasis diagnostic method in endemic areas of Venezuela. MATERIALS AND METHODS...