The accuracy of three force fields for predicting the physical properties of dimethylnitramine (DMNA) has been investigated by using molecular dynamics simulations. The Sorescu, Rice, and Thompson (SRT) (J. Phys. Chem. B 1997, 101, 798) rigid-molecule, flexible generalized AMBER (J. Comput. Chem. 2004, 25, 1157), and Smith et al. flexible force fields (J. Phys. Chem. B 1999, 103, 705) were test...

The universal genetic code includes three codons which signal polypeptide chain termination. These termination or nonsense codons are UAG (amber), UAA (ochre), and UGA (opal). Usually, Escherichia coli and other procaryotic cells do not contain transfer ribonucleic acids (tRNAs) which recognize these codons. However, such tRNAs can be created by suppressor mutations in tRNA genes, generating tR...

The sequence dependence of RNA energetics is important for predicting RNA structure. Hairpins with C(n) loops are consistently less stable than hairpins with other loops, which suggests the structure of C(n) regions could be unusual in the "unfolded" state. For example, previous nuclear magnetic resonance (NMR) evidence suggested that polycytidylic acid forms a left-handed helix. In this study,...

NinJo ist eines der weltweit größten meteorologischen Visualisierungssysteme. Die Software wird seit 2007 operationell in der Wetterberatung eingesetzt. Für die agrarmeteorologische Beratung im Deutschen Wetterdienst (DWD) wurde für NinJo eine Komponente zur Visualisierung agrarmeteorologischer Daten entwickelt, der sogenannte AMBER-Layer. Dieser Artikel stellt den AMBERLayer innerhalb des Gesa...

We synonymize Hellichiella pugach Perkovsky & sukhomlin, 2015 with Gre­ niera yankovskyi Perkovsky & sukhomlin, 2015 (stegopterniini) based on the holotype and female paratype of H. pugach (stegopterniini). Hellichiella po­ lessica n. sp. differs from Hellichiella oligocenica (rubtsov, 1936) (the only species of Hellichiella known in Baltic amber) by its smaller body, narrower head, 11-segmente...

The isolation of one amber mutation in malQ, one ochre mutation in malP, and seven amber mutations in malT is reported. A study of their phenotypic expressions in the presence of the amber suppressor su(III) and the ochre suppressor su(c) suggests that (i) malQ is the structural gene for amylomaltase; (ii) malQ and the structural gene for maltodextrin phosphorylase, malP, belong to the same ope...

Rare or narrowly distributed species may be threatened by stressors to which they have never been exposed or for which data are very limited. In such cases the species response cannot be predicted on the basis of directly measured data, but may be inferred from the response of one or more appropriate surrogate species. Here, I propose a practical way to use the stressor response of one or more ...

The goal of this research was to study expression of the tRNAT, gene family in the nematode Caenorhabditis elegans. Eight of the 12 members, identical in coding sequence, were previously recovered as amber suppressor alleles, and suppression efficiency of amber mutations in different genes was different for individual suptRNAT, genes (Kondo, et al., 1990; 1988). The results suggested that membe...

Microscapha andrzeji sp. nov., the first fossil representative of the genus is described from Eocene Baltic amber. An updated list of fossil Melandryidae (Coleoptera: Tenebrionoidea) is provided. The presence of Microscapha within Baltic amber suggests some potential for palaeoenvironmental inferences based on the melandryid assemblage within the deposit.

An amber suppressor variant of Bacillus subtilis tyrosyl-tRNA was constructed and placed under control of the isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible Pspac promoter. Addition of IPTG resulted in a 50-fold increase in the expression of an rpsD-lacZ fusion containing a UAG amber codon. This system permitted isolation of a conditional lethal mutant which required IPTG for growth.