The present article draws a general picture of non-conventional methods for biomolecules immobilization. The technologies presented are based either on original solid supports or on innovative immobilization processes. Polydimethylsiloxane elastomer will be presented as a popular immobilization support within the biochip developer community. Electro-addressing of biomolecules at the surface of ...

One of the most important goals in molecular biology is to elucidate the relationship among sequence, structure, function and properties of biomolecules. Such knowledge would enable us to design the modifications of biomolecules for particular functions, and drugs to modify the function and property of biomolecules. Now, the number of entries in the Protein Data Bank (PDB) is over 10,000. These...

A Gaussian accelerated molecular dynamics (GaMD) approach for simultaneous enhanced sampling and free energy calculation of biomolecules is presented. By constructing a boost potential that follows Gaussian distribution, accurate reweighting of the GaMD simulations is achieved using cumulant expansion to the second order. Here, GaMD is demonstrated on three biomolecular model systems: alanine d...

Background Understanding the biology of platelets is essential for the management of thrombotic diseases like stroke and cardiovascular disease. Platelets store ATP and ADP biomolecules and secrete them in response to thrombotic stimuli. However, patient-to-patient variability in storage of these biomolecules in platelets is poorly studied [1]. This study assesses ATP and ADP concentration in p...

Biomolecules such as proteins, on silicon based surfaces are of extreme importance in various applications including microfabricated silicon implants, in the fabrication of microdevices with protein compounds (e.g., biosensors), and therapeutics. Morphology of silicon based surfaces with and without biomolecules and their adhesion with the substrate govern performance and reliability of the bio...

objective(s): microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.   materials and methods: in this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-l-lysine (pll). some of the aldehyde groups of the activated agaro...

Many processes in the body are effected and regulated by highly specialized protein molecules: These molecules certainly deserve the name "biochemical nanomachines". Recent progress in single-molecule experiments and corresponding simulations with supercomputers enable us to watch these "nanomachines" at work, revealing a host of astounding mechanisms. Examples are the fine-tuned movements of t...

Recent advances in single-molecule detection and single-molecule spectroscopy at room temperature by laser-induced fluorescence offer new tools for the study of individual macromolecules under physiological conditions. These tools relay conformational states, conformational dynamics, and activity of single biological molecules to physical observables, unmasked by ensemble averaging. Distributio...