To improve our understanding of the prostate-specific antigen (PSA) antigenic regions, we studied the association targets of one anti-PSA polyclonal antibody and 10 anti-PSA monoclonal antibodies (mAbs). We also examined the ability of the mAbs to inhibit PSA enzymatic activity and block the association of PSA with alpha 1-antichymotrypsin (ACT). Linear epitope mapping with a polyclonal antibod...

the leishmania major parasite surface antigen-2 (psa-2) is a family of glycoinositol phospholipids anchored glycoprotoins expressed in both promastigotes and amastigotes. promastigote psa-2 comprises three polypeptides with approximate molecular weight of 96, 80 and 50 kda. amastigote express a distinct but closely psa-2 polypeptide with molecular weight of 50 kda. in this study fusion of sp2/0...

INTRODUCTION Prostate-specific antigen (PSA) is a widely used tumor marker in the detection and follow-up of adenocarcinoma of the prostate. Selection of candidates for prostate biopsies is hampered by the lack of specificity resulting in a large number of unnecessary biopsies. The intention of our study was to compare the percent free PSA (f-PSA; Hybritech Tandem-R) with total PSA and age-spec...

background prostate is an important male reproductive system gland and its disorders can affect men's quality of life and health. prostatitis, benign prostatic hyperplasia (bph), and prostate adenocarcinoma are major disorders that can be found in all men in different ages. objectives the aim of this study was to investigate the association of diet with serum prostate specific antigen (psa) lev...

The landscape of prostate cancer has changed since the appearance of the first prostate-specific antigen (PSA) assay. PSA testing has gained rapid recognition since M. Kuriyama et al. (1 ) of Roswell Park first reported clinical studies using an enzyme immunoassay with anti-PSA rabbit antibody. Mikolajczyk and coworkers (2, 3) reported the presence of several free PSA isoforms and the potential...

In the quarter-century since its discovery (1 ), prostatespecific antigen (PSA) has been recognized as the most effective tumor marker for prostate cancer and has unquestionably played an important role in the diagnosis and clinical management of this disease. Prostate cancer remains the leading cancer site in men, with 179 300 new cancer cases estimated by the American Cancer Society for 1999,...

background: prostate specific antigen (psa) is a protein currently used in vconjunction with the digital rectal examination to detect and guide management of prostate cancer. there are several causes of increase in serum psa including urological manipulations, medications, prostatic diseases, prostate cancer, prostate inflammation or infection, etc. some studies had reported cystoscopy to cause...

The importance of insulin-like growth factor 1 (IGF-1) in human serum for the early diagnosis of prostate cancer is controversial. The IGF-1/PSA ratio may improve the performance of prostate specific antigen (PSA) as a prostate cancer marker. IGF-1, along with PSA and free PSA concentration, was measured in the serum of 34 patients with prostate cancer and in 131 patients with benign prostatic ...

Fucosylation is an important oligosaccharide modification associated with cancer and inflammation. We investigated whether urinary fucosylated PSA (Fuc-PSA) levels could be used for the detection of high Gleason score prostate cancer. Urine samples were collected from men with abnormal digital rectal examination findings or elevated serum PSA levels, before prostate biopsy. Lectin-antibody ELIS...