N-terminal acetylation (NTA) catalysed by N-terminal acetyltransferases (Nats) is among the most common protein modifications in eukaryotes, but its significance is still enigmatic. Here we characterize the plant NatA complex and reveal evolutionary conservation of NatA biochemical properties in higher eukaryotes and uncover specific and essential functions of NatA for development, biosynthetic...

A previous microarray analysis suggested that multicopy yccH, encoding a function-unknown response regulator, enhances expression of natAB, which encodes a two-gene ATP-binding cassette transporter involved in the extrusion of sodium ions. The two-component regulatory system YccG-YccH was therefore renamed NatK-NatR. Here, this observation was confirmed by a lacZ fusion analysis using a strain ...

bei der Modellierung der Chromatinstruktur. Abstract Abstract N α-acetylation, one of the most abundant eukaryotic protein modifications, is catalyzed by N-terminal acetyltransferases (NATs). NatA, the major NAT in Saccharomyces cerevisiae, consists of the subunits Nat1, Ard1 and Nat5 and is necessary for the assembly of repressive chromatin structures at the silent mating type loci and telomer...

The human NatA protein N(alpha)-terminal-acetyltransferase complex is responsible for cotranslational N-terminal acetylation of proteins with Ser, Ala, Thr, Gly, and Val N termini. The NatA complex is composed of the catalytic subunit hNaa10p (hArd1) and the auxiliary subunit hNaa15p (hNat1/NATH). Using immunoprecipitation coupled with mass spectrometry, we identified endogenous HYPK, a Hunting...

Nata de coco is a type of beverage component is a cellulose compound produced from coconut water through a process that involves microbial fermentation known as Acetobacter xylinum. To increase the economic value, shelf life, and the benefits of diversification do nata product of nata de coco wet a fiber-rich instant drink of nata de coco. This research used Completely Randomized Design (CRD) a...

Nata is a bacterial cellulose obtained from the fermentation of Acetobacter xylinum. It often found in desserts as health supplement because its high fiber content. not only produced coconut water, known nata-de-coco but can also be other sources, such wastewater rice washing, called nata-de-leri. This study aimed to optimize parameters (nata-de-leri) by statistical methods improve production. ...

Nata de coco business development has good economic potential. is a low-calorie snack whose fiber can aid physiological processes. It an appealing aroma and be prepared in variety of flavors, it lasts long time. The nata was chosen as the training material because with simple processing technologies, making to execute for students. purpose program SMA Kesatrian 1 Semarang students strengthen th...

In eukaryotes, N-terminal acetylation is one of the most common protein modifications involved in a wide range of biological processes. Most N-acetyltransferase complexes (NATs) act co-translationally, with the heterodimeric NatA complex modifying the majority of substrate proteins. Here we show that the Huntingtin yeast two-hybrid protein K (HypK) binds tightly to the NatA complex comprising t...

The X-linked lethal Ogden syndrome was the first reported human genetic disorder associated with a mutation in an N-terminal acetyltransferase (NAT) gene. The affected males harbor an Ser37Pro (S37P) mutation in the gene encoding Naa10, the catalytic subunit of NatA, the major human NAT involved in the co-translational acetylation of proteins. Structural models and molecular dynamics simulation...

N(α)-Acetyltransferases (NATs) cause the N(α)-acetylation of the majority of eukaryotic proteins during their translation, although the functions of this modification have been largely unexplored. In yeast (Saccharomyces cerevisiae), four NATs have been identified: NatA, NatB, NatC, and NatD. In this study, the N(α)-acetylation status of ribosomal protein was analyzed using NAT mutants combined...