background: in this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. materials and methods: human umbilical cord blood-derived mesenchymal stem cells were first cultured in dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incub...

Parkinson disease is a neurological disorder accompanied by degeneration of dopaminergic system and neuronal loss. Recently studies have focused on stem cells therapy, therefore the goal of this study was to investigate the effect of adipose derived stem cells grafting, on locomotion and imbalance in Parkinson model of rats. Twenty-four male Wistar rats weighing 200-250 g were divided into thre...

The brain is an important organ that controls all sensory and motor actions, memory, and emotions. Each anatomical and physiological modulation in various brain centers, results in psychological, behavioral, and sensory-motor changes. Alcohol and addictive drugs such as opioids and amphetamines have been shown to exert a great impact on brain, specifically on the hippocampus. Emerging evidence ...

Background: Cancer stem-like cells (CSCs) play a crucial role in the initiation, progression, and recurrence of cancer. Evidence indicates that the high linear energy transfer (LET) carbon ion beam is more effective against CSCs than the conventional X-ray beam. Carbon ion radiotherapy is considered as a promising cancer strategy, however, information about whether, or not, new CSCs are induced...

royan b 1 stem cell can be differentiated to specialized cell types including cardiomyocytes. this developmental change is accompanied with expression of various k + channel types. the aim of this study was to detect functional expression of k + currents from stem cell stage and one week and two weeks after differentiation into cardiomyocyte. mouse stem cell derived cardiomyocytes (es-cardiomyo...

conclusions our data suggest that preconditioning with 5 μm h2o2 + 0.5% fbs and 10 μm h2o2 + 5% fbs improved cell proliferation and viability. however, the mechanisms related to protective properties of preconditioning and using this strategy in stem cell therapy requires more research. background mesenchymal stem cells (mscs) survival decreased following in vivo injection and its application h...

background: due to increasing clinical demand for adipose tissue, a suitable cell for reconstructive adipose tissue constructs is needed. in this study, we investigated the ability of human endometrial-derived stem cells (enscs) as a new source of mesenchymal stem cells to differentiate into adipocytes. enscs are the abundant and easy available source with no immunological response, for cell re...

The aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 1-2 days in DMEM medium. The inner cell masses formed...