background: visceral leishmaniasis (vl) or kala-azar is a parasitic disease caused by the species of leishmania donovani complex. mediterranean type of the disease is endemic in some parts of iran and more than 95% of cases were reported in children up to 12 years of age. this study was performed to determine the seroprevalence of vl in the rural areas of dashti district from bushehr province. ...

background: this study aimed to determine the seroprevalence of canine visceral leishmaniasis in meshkin-shahr district as endemic areas of human visceral leishmaniasis (hvl) for presenting control program of hvl to health authorities.  methods: a seroepidemiological study to determine seroprevalence of canine visceral leishmaniasis (cvl) among owner­ship dogs using direct agglutination tests (...

b a c k g r o u n d & aim: visceral leishmaniasis (vl) or kala-azar is a parasitic disease caused by the species of leishmania donovani complex. mediterranean type of disease is endemic in some parts of iran and more than 95% of seropositivity cases were reported in children up to 12 years of age. a cross-sectional study was conducted to determine the seroprevalence of vl in nomadic tribe’s pop...

The performance of a direct agglutination test for the detection of toxoplasma specific IgG immunoglobulin was compared with that ofthe latex agglutination test. The direct agglutination test was less sensitive but more specific than the latex agglutination test. Quantitative results were not directly comparable, reflecting the different antibody profiles detected in each assay. The direct aggl...

OBJECTIVE To compare the performance of the direct agglutination test and rK39 dipstick for the diagnosis of visceral leishmaniasis. DATA SOURCES Medline, citation tracking, January 1986 to December 2004. Selection criteria Original studies evaluating the direct agglutination test or the rK39 dipstick with clinical visceral leishmaniasis as target condition; adequate reference classification;...

background: new cases of visceral leishmaniasis (vl) have been reported recently in some parts of mazandaran province, north of iran where the first human case of  vl was reported in 1949. this study aimed to determine the present status of leishmania infantum infection among humans and domestic dogs using serological and molecular methods in central parts of mazandaran province. methods: in th...

Titers in a direct agglutination test using Formalin-fixed toxoplasma tachyzoites were significantly higher in patients with acquired immunodeficiency syndrome with toxoplasmic encephalitis (TE) than in those without TE. In contrast, when fresh (not fixed) tachyzoites were used, the titers did not differ from those in patients without TE. The direct agglutination test using Formalin-fixed tachy...

It is technically impractical for many clinical laboratories to use the direct immunofluorescence assay for identifying and serogrouping clinical isolates of Legionella. We compared the results obtained with the direct immunofluorescence assay with the results of a simple and less-demanding slide agglutination test for identifying 15 serogroups representing seven Legionella species. The slide a...

The Coombs test (Coombs, Mourant, and Race, 1945a, 1945b, 1946) has become popular as a sensitive and reliable method of detecting incomplete antibodies. The essential reagent is the serum of an animal (usually a rabbit) which has been immunized with human globulin. Potent rabbit sera sometimes show a prozone phenomenon (Wootton, 1950; Van Loghem, Kresner, Coombs, and Fulton Roberts, 1950). The...

The BrucellaCapt test is an immunocapture agglutination test suggested as a possible substitute for the Coombs test in the diagnosis of human brucellosis. Here it is compared with classical tests using 321 samples from 48 patients with brucellosis (6.9 +/- 1.7 samples per patient), including 20 patients with focal disease and 8 patients with a total of 9 relapse episodes (mean follow-up, 18 mon...