A UHPLC-(ESI+)-MS/MS method was developed and validated for the simultaneous determination of glucoraphanin (GR), sulforaphane (SF), sulforaphane-glutathione (SF-GSH), sulforaphane-cysteine (SF-CYS), sulforaphane-N-acetyl-cysteine (SF-NAC), in a range biological materials (digesta, cell culture medium, lysates, urine) with no sample pretreatment. The limit detection (LODs) ranged from 0.03 to 1...

BACKGROUND The accuracy of synovial fluid (SF) white cell count (WCC) and polymorphonuclear (PMN) cell evaluation for predicting prosthetic joint infection (PJI) at the total hip arthroplasty (THA) or total knee arthroplasty (TKA) site is unknown. Therefore, we performed a meta-analysis to summarize the diagnostic validity of SF-WCC and SF-PMN for diagnosing PJI. METHODS The MEDLINE, EMBASE, ...

A. Overview of steroidogenesis B. SF-1 and the regulation of steroidogenesis C. Cloning and structural characterization of SF-1 1. Structural features of SF-1 2. Multiple transcripts are encoded by the gene encoding SF-1 3. The gene encoding SF-1 is evolutionarily conserved in vertebrates and invertebrates III. Characterization of Sites of SF-1 Expression and Identification of Its Target Genes ...

We report the draft genome sequences of marine RNA viruses SF-1, SF-2, and SF-3, which were assembled from metagenomic sequencing of organisms in San Francisco wastewater. These viruses were most closely related to marine RNA virus JP-B and algae viruses.

Scatter factor (SF) (also known as hepatocyte growth factor [HGF]) is a fibroblast-derived cytokine that stimulates motility, proliferation, and morphogenesis of epithelia. SF may play major roles in development, repair, and carcinogenesis. However, the physiologic signals that regulate its production are not well delineated. We found that various human tumor cell lines that do not produce SF s...

Naturally occurring sulforaphane (SF) has been extensively studied for cancer prevention. However, little is known as to which organs may be most affected by this agent, which impedes its further development. In the present study, SF was administered to rats orally either in a single dose or once daily for 7 d. Tissue distribution of SF was measured by a HPLC-based method. Glutathione S-transfe...

Four pathogenic Vibrio species were isolated from three diseased black rockfish Sebastes schlegeli in Yantai, Shandong Province, China. The strains identified based on physiological and biochemical characteristics 16S rDNA sequencing named SF-2, SF-3, SF-5, SF-6, respectively. SF-2 was scophthalmi, SF-3 V. harveyi, SF-5 alginolyticus, SF-6 parahaemolyticus. This is the first time that scophthal...

OBJECTIVE A low sexual function (SF) has been reported in patients with colorectal cancer. However, research often focusses on clinical predictors of SF, hereby omitting patients' subjective evaluation of SF [i.e., the quality of sexual life (QoSL)] and psychosocial predictors of SF and QoSL. In addition, research incorporating a biopsychosocial approach to SF and QoSL is scarce. Therefore, thi...