113P Spatial transcriptomic profiling of real-world, archival, resected pancreatic ductal adenocarcinomas

Pancreatic ductal adenocarcinoma (PDAC) tissue poses a unique challenge for bulk-transcriptomic gene expression, due to their relative stromal density and abundance of inherent ribonuclease enzymes. Spatial transcriptomic (ST) profiling is an established technique explore the molecular architecture human cancers. The use these ST technologies has evolved from primary development in fresh-frozen tissue, formalin-fixed paraffin-embedded (FFPE) samples. This created interest applying archival PDAC resection specimens. A selection FFPE samples surgically resected pancreatic adenocarcinomas underwent 10x Genomics Visium Gene Expression analysis. comprised: 2x classical sub-type, squamous treatment naïve, neoadjuvant treated with chemotherapy. panel 18,000 probes was used i.e Whole Human Transcriptome. Differential expression cluster analysis performed characterise tumour microenvironment identify immune components. Comparison made between prior bulk-transcriptomics on ranged RNA integrity; no sample had more than 40% measured fragments > 200 nucleotides length indicating substantial fragmentation. mean number genes identified per 17, 871. 55μm area 4,617. Clustering models demonstrated distinct cell populations correlation histopathological architecture. These data suggest that can be yield meaningful information many variants PDAC. Applying specimens may allow creation spatial atlas architecture; as well adjunct bulk single-cell interrogation. As therapeutic targeting based subtyping develops, provide valuable insight into heterogeneity signalling within