14-3-3-Pred: improved methods to predict 14-3-3-binding phosphopeptides

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14-3-3-Pred: improved methods to predict 14-3-3-binding phosphopeptides

MOTIVATION The 14-3-3 family of phosphoprotein-binding proteins regulates many cellular processes by docking onto pairs of phosphorylated Ser and Thr residues in a constellation of intracellular targets. Therefore, there is a pressing need to develop new prediction methods that use an updated set of 14-3-3-binding motifs for the identification of new 14-3-3 targets and to prioritize the downstr...

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The 14-3-3 family of proteins mediates signal transduction by binding to phosphoserine-containing proteins. Using phosphoserine-oriented peptide libraries to probe all mammalian and yeast 14-3-3s, we identified two different binding motifs, RSXpSXP and RXY/FXpSXP, present in nearly all known 14-3-3 binding proteins. The crystal structure of 14-3-3zeta complexed with the phosphoserine motif in p...

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ژورنال

عنوان ژورنال: Bioinformatics

سال: 2015

ISSN: 1367-4803,1460-2059

DOI: 10.1093/bioinformatics/btv133