3-D stimulated emission depletion microscopy with programmable aberration correction
نویسندگان
چکیده
منابع مشابه
Stimulated emission depletion microscopy with optical fibers
Imaging at the nanoscale and/or at remote locations holds great promise for studies in fields as disparate as the life sciences and materials sciences. One such microscopy technique, stimulated emission depletion (STED) microscopy, is one of several fluorescence based imaging techniques that offers resolution beyond the diffractionlimit. All current implementations of STED microscopy, however, ...
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In recent years, various methods have been proposed to increase the resolution of fluorescence microscopy beyond the diffraction limit of light. Amongst them, Stimulated Emission Depletion (STED) microscopy, which relies on a purely physical concept of fluorescence depletion could, in theory, achieve unlimited resolution independent of the wavelength of light. In this chapter, we review the fun...
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With recent developments in microscopy, such as stimulated emission deletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we analyse how the interplay between shot noise, thermal noise, the saturation intensity of a fluorescent label, and the damage threshold of the sample changes the maximum resolution of...
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ژورنال
عنوان ژورنال: Journal of Biophotonics
سال: 2013
ISSN: 1864-063X
DOI: 10.1002/jbio.201300041