5-Bromodeoxyuridine-Induced Differentiation of a Neuroblastoma

5-bromodeoxyuridine-induced differentiation of a neuroblastoma.

5-Bromodeoxyuridine induces the differentiation of mouse neuroblastoma C1300 to cells that morphologically resemble mature neurons. The induced differentiation can take place in the absence of DNA synthesis. This suggests that the halogenated pyrimidine need not be incorporated into DNA to alter the phenotype of the cell.

The effect of 5-bromodeoxyuridine on cell division and differentiation of preimplantation mouse embryos.

Mouse embryos exposed to concentrations of 5-bromodeoxyuridine (BUdR) ranging from 0-01 to 1-0 mug/ml in vitro for two days from the 8-cell stage exhibit a concentration-dependent decrease in the frequency of normal blastocysts and decrease in average cell number per embryo. A 20-h exposure was adequate to achieve the full BUdR response. Both effects were eliminated in the presence of excess th...

In vitro differentiation of a mouse neuroblastoma.

Mouse tumor C1300 has been established in tissue culture. The cells have a round cell morphology in both the subcutaneous tumor and in suspension culture. However, when given a surface on which to attach, they send out processes up to 3 mm in length and assume the morphology of mature neurons. The attached cells are stained by the Bodian silver procedure for neurons, whereas the cells grown in ...

5-Bromodeoxyuridine-induced formation of virus-like particles in Naegleria gruberi EGs.

Exposure of axenic cultures of the amoeboflagellate Naegleria gruberi EGs to the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU) resulted in the induction of virus-like particles (VLP) and various structures associated with their development and presumed transmission. Previously, VLP induction could be accomplished only by growing amoebae in the presence of living bacteria as a food source. A...

Effect of 5-bromodeoxyuridine on the Experimentally Induced Radioresistance of Escherichia Coli.