A-142 Comparison of Alinity c Hemoglobin A1c Immunoassay with Premier Hb9210 Automated HPLC Assay: Very Few Patients May be Outside Reportable Range Using Alinity c.

Abstract Background Hemoglobin A1c (HbA1c; glycosylated hemoglobin) is used for diagnosis and monitoring of patients with diabetes. In the clinical laboratory both immunoassays high-performance liquid chromatography (HPLC) based tests are available such measurement. We use Premier Hb9210 analyzer (HPLC method; Trinity Biotech, Jamestown, NY) measuring HBA1c in whole blood. As our transitioning to Abbott chemistry systems, we compared HbA1c values obtained by Alinity c Hb9210. Methods The on boronate affinity high performance output 66 sec. analytical measurement range 3.8 18.5% although as low 2% can be reported but result may not accurate. assay measures total hemoglobin (enzymatic assay) blood then calculates %HbA1c. this 4 14% HbA1c. Specimens cannot diluted Results has excellent precision (0.4% at 5.1% 0.25% Hb 9.4%). contrast, HB9210 1.89% 5.5% 1.81% 8.4%. However, HB9219 slightly wider assay. Plotting results x-axis corresponding assay, observed following regression equation: y = 0.9499 x + 0.1108 ( n 50, 0.99). 95% confidence interval slope was 0.9241 0.9757. Rarely, get specimen where between 4.0% (42 specimens out 52 803 2/7/22 2/7/23; 0.08%) or above (174 802; same period; 0.33%) these would produce any recently 2.4 % a sickle cell patient (reported <3.8% per lab policy). Discussion Our indicates that immunoassay showed comparable HPLC method. very few exceed due uncontrollable Because diluted, real value could measured using Conclusions conclude viable alternative laboratory. (sickle trait, uncontrolled diabetes), outside c, requiring analysis.