A COLORIMETRIC METHOD FOR THE DETERMINATION OF CHYMOTRYPSIN ACTIVITY
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چکیده
منابع مشابه
A Colorimetric Method for the Determination of Chymotrypsin Activity * by Beat
The L isomers of esters, amides, and hydrazides of the general formula RCONHCHRICORz where R = CHS-, CsHb---, etc., RI = CsH&H2-, p-HO-&H&H-, etc., and Rz = -0CH3, -NH*, or -NHNH2, are known to be hydrolyzed by chymotrypsin (l-6). It has now been found that the corresponding hydroxamides, RCONHCHRICONHOH, where R1 = CsH&Hz--, are also hydrolyzed by this enzyme (Table I). Aside from the obvious ...
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The structural specificity requirements of chymotrypsin have been well defined and reviewed by several investigators (l-3). As in the case of carboxypeptidase (4), the specific amino acid required in a substrate for chymotrypsin is tyrosine, tryptophan, or phenylalanine, in order of decreasing susceptibility of their corresponding ester or peptide derivatives. The enzyme is reported to attack, ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1950
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)51164-4