A DISSECTION TECHNIQUE FOR EMBRYO SACS

Donor corneae for preservation. A modified dissection technique.

THE simple method of dissecting a corneo-scleral button from the donor eye described below is useful in maintaining a uniform plane of dissection for lamellar grafts because a dark background is provided by the uveal tissue kept in situ with the preserved donor cornea. After enucleation the eye is washed with sterile normal saline, kept immersed in 1: 5000 solution of Merthiolate for 5 minutes,...

Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo.

Avian embryos are a commonly used model system for developmental studies, but monitoring of physiological parameters such as heart rate (HR) and movement in ovo poses a challenge to researchers. These are also increasingly common research objectives for ecological and embryo behavior studies in oviparous species. We therefore explored the validity of a new digital egg-monitoring system for the ...

An alternative technique for cannulation in type A dissection.

In a recent issue, there was a Great Debate article about cannulation techniques in patients with type A dissection complicated by cerebral malperfusion [1]. Paul P. Urbanski argues for cannulation of the common carotid artery by using a separate incision along the medial margin of the sternocleidomastoid muscle. Jean Bachet gives arguments for the right axillary artery as cannulation site by a...

Transcript profiles of maize embryo sacs and preliminary identification of genes involved in the embryo sac–pollen tube interaction

The embryo sac, the female gametophyte of flowering plants, plays important roles in the pollination and fertilization process. Maize (Zea mays L.) is a model monocot, but little is known about the interactions between its embryo sac and the pollen tube. In this study, we compared the transcript profiles of mature embryo sacs, mature embryo sacs 14-16 h after pollination, and mature nucelli. Co...

Dissection technique in lamellar keratoplasty.