A flow cytometric assay for intracellular nonprotein thiols using mercury orange

Rapid Flow Cytometric Method for Measuring Mitochondrial Membrane Potential, Respiratory Burst Activity, and Intracellular Thiols of Human Whole Blood Leukocytes

Identifying key perturbations of redox regulatory mechanisms may help to understand stress-related disease processes. Currently, flow cytometry allows the functional characterisation of redox changes that occur in different cellular compartments: changes in mitochondrial membrane potential, respiratory burst activity, and intracellular thiol levels are measurable in live white blood cells. Howe...

A rapid and sensitive intracellular flow cytometric assay to identify Theileria parva infection within target cells.

Theileria parva is an intracellular protozoan parasite transmitted by ticks that causes a fatal lymphoproliferative disease of cattle known as East Coast Fever. Vaccination against the disease currently relies on inoculation of the infective sporozoite stage of the parasite and simultaneous treatment with long-acting formulations of oxytetracycline. Sporozoites are maintained as frozen stabilat...

Hydrogen peroxide-induced epithelial injury: the protective role of intracellular nonprotein thiols (NPSH).

Injury to the alveolar region is a hallmark of the adult respiratory distress syndrome (ARDS) whereas injury to the epithelium of the conducting airways is a characteristic of asthma. Reactive oxygen species have been implicated as mediators of lung injury in both of these conditions. We have investigated the relationship between intracellular nonprotein thiols (NPSH), and the release of the cy...

Rapid and Thiol-Specific High-Throughput Assay for Simultaneous Relative Quantification of Total Thiols, Protein Thiols, and Nonprotein Thiols in Cells

Thiol groups in biological molecules play a significant role in various physiological functions and pathological conditions. Thiols are divided into two major groups: protein thiols and nonprotein thiols. Numerous methods have been reported for thiol assays. Most of these methods have been developed for glutathione, the principal nonprotein thiol, despite the fact that cellular protein thiols a...

Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis