A high-throughput integrated microfluidics method enables tyrosine autophosphorylation discovery
نویسندگان
چکیده
منابع مشابه
High-throughput metabolomics enables biomarker discovery in prostate cancer
Prostate cancer (PCa) is the most frequently diagnosed cancer and the second leading cause of cancer death among men in the world. Therefore, it is important to discover new biomarkers for the diagnosis of PCa, preferably noninvasive ones. Metabolomics can be used to analyse the entire metabolic profile of a biological system and discover potential biomarkers. Advances in liquid chromatography-...
متن کاملDroplet microfluidics for high-throughput biological assays.
Droplet microfluidics offers significant advantages for performing high-throughput screens and sensitive assays. Droplets allow sample volumes to be significantly reduced, leading to concomitant reductions in cost. Manipulation and measurement at kilohertz speeds enable up to 10(8) samples to be screened in one day. Compartmentalization in droplets increases assay sensitivity by increasing the ...
متن کاملHigh-throughput injection with microfluidics using picoinjectors.
Adding reagents to drops is one of the most important functions in droplet-based microfluidic systems; however, a robust technique to accomplish this does not exist. Here, we introduce the picoinjector, a robust device to add controlled volumes of reagent using electro-microfluidics at kilohertz rates. It can also perform multiple injections for serial and combinatorial additions.
متن کاملA high-throughput cellulase screening system based on droplet microfluidics.
A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% pur...
متن کاملMicrofluidics for cell-based high throughput screening platforms - A review.
In the last decades, the basic techniques of microfluidics for the study of cells such as cell culture, cell separation, and cell lysis, have been well developed. Based on cell handling techniques, microfluidics has been widely applied in the field of PCR (Polymerase Chain Reaction), immunoassays, organ-on-chip, stem cell research, and analysis and identification of circulating tumor cells. As ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Communications Biology
سال: 2019
ISSN: 2399-3642
DOI: 10.1038/s42003-019-0286-9