A method for coating multiple slides for light microscopic autoradiography.
نویسندگان
چکیده
منابع مشابه
Electron Microscopic Autoradiography
An improved method has been developed for the preparation of autoradiographs for electron optical study. The refinement lies principally in the routine production of uniformly thin layers of photographic emulsion over the tracer-labeled cell sections. This is accomplished by means of a centrifugal spreading mechanism. Maize root tips grown in the presence of H(3)-thymidine were examined electro...
متن کاملElectron Microscopic Autoradiography
Iodide is concentrated from the blood by the thyroid gland; after oxidation it is incorporated in tyrosyl residues of the thyroglobulin molecule. Thus the halogen is stored as a component of thyroglobulin in the colloid of the thyroid follicle. Secretion of thyroid hormone is considered to require proteolysis of the thyroglobulin and release of iodinated thyronines from the base of the acinar c...
متن کاملQuantitative Electron Microscopic Autoradiography
Quantitative electron microscopic autoradiography was used for comparing the binding of labeled somatostatin-14 (S-14) and somatostatin-28 (S-28) to islet cells. Monolayer cultures of rat islet cells were incubated with ['251-Tyr"jS-14 (S-14) or [125I-Leu8, D-Trp22, TyreJS-28 (S-281) in the presence or absence of excess unlabeled peptides. Autoradiographic grains (ARG) associated with individua...
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Objective(s): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules. Materials and Methods: In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated aga...
متن کاملLight microscopic autoradiography for study of early changes in the distribution of water-soluble materials.
An approach using autoradiography for the study of early changes in the distribution of water-soluble materials and the chemography involved was investigated. Radioactive calcium chloride (45Ca) was injected into the iliac vein of a rat. Ten seconds after the injection the rat was frozen in hexane (-90 degrees C). The frozen rat was embedded in 5% sodium carboxymethyl cellulose and blocked in t...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1977
ISSN: 0022-1554,1551-5044
DOI: 10.1177/25.9.333021