A microfluidic oligonucleotide synthesizer
نویسندگان
چکیده
منابع مشابه
A microfluidic oligonucleotide synthesizer
De novo gene and genome synthesis enables the design of any sequence without the requirement of a pre-existing template as in traditional genetic engineering methods. The ability to mass produce synthetic genes holds great potential for biological research, but widespread availability of de novo DNA constructs is currently hampered by their high cost. In this work, we describe a microfluidic pl...
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BACKGROUND Oligonucleotides (ONs) have shown great promise as therapeutic agents for various diseases. It is necessary to provide a protocol for preparation of ON-loaded lipid nanoparticles (LNPs) in a reproducible manner on a laboratory scale. MATERIALS AND METHODS A 3-inlet microfluidic (MF) chip-based device was used to synthesize LNPs at the lipid/ON ratio of 10/1 (w/w) and at flow rates ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2010
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkq092