A NEGATIVE STAINING METHOD FOR CELL-ASSOCIATED VIRUS
نویسندگان
چکیده
منابع مشابه
A Negative Staining Method for Cell-associated Virus
The virus host-cell relationship has been studied in the electron microscope almost entirely by the thin sectioning technique. This method has given information about the sites of virus production within the cell and the appearance of virus particles at various stages in the growth cycle (1, 2). I t does not, however, permit high resolution studies on the substructure of individual particles or...
متن کاملNegative Staining of Thinly Spread Cells and Associated Virus
The first demonstration of intracytoplasmic virus by negative staining was that of Horne and Nagington (9) who subjected poliovirus-infected cells to freezing and thawing and negatively stained a suspension of the fragments. Dales (5) examined the attachment of some viruses to the cell surface by the negative staining of whole mounts of cells subjected to osmotic shock. Whittaker and Horne (15)...
متن کاملRapid semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy.
A rapid, semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy is presented. Results obtained by this method are compared with results obtained by the pseudoreplica method and a measureddrop method. A figure is presented which represents the limit of detectability for virus by negative staining.
متن کاملA new method for staining leprosy bacilli.
To a paper on a new method for staining tubercle bacilli with Nachtblau which the writer published in 1941, Reenstierna added a short note (1), together with a plate, on its use in staining leprosy bacilli in nasal smears, films of blood, and sections of lepromata. As was the case with tubercle bacilli, the bacilli of leprosy were found to take a dark-blue color, to appear larger and more disti...
متن کاملA Staining Method for Corynebacterium diphtheriae.
The following method is recommended for the staining of Corynebacterium diphtheriae. It provides a good contrast between the body of the organism and the granules in it. Cover the fixed and dried film with Loeffler's methylene blue, leave for five minutes, wash with water, decolorize rapidly with 1/1000 sulfuric acid. The acid should not stay on the film for more than a few seconds, wash with w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1963
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.16.3.616