A new minisatellite probe shows highly polymorphic hybridization pattern in human
نویسندگان
چکیده
منابع مشابه
A highly polymorphic locus in human DNA.
A locus in the human genome, not associated with any specific gene, has been found to be a site of restriction fragment length polymorphism. The polymorphism was found by hybridizing a 16-kilobase-pair segment of single-copy human DNA, selected from the human genome library cloned in phage lambda CH4A, to a Southern transfer of total human DNA digested with EcoRI. DNAs from a number of individu...
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Source/Description: NB17 is a 0.7 kb HindlO-EcoRI fragment isolated from two pooled chromosome 22 specific libraries (AT-CC # 57733 and ATCC # 57714) and was subcloned into pUC9. Polymorphism: Bgin or TaqI digestion of genomic DNA and hybridization with the probe detects a two allele polymorphism: Bgin (Al: 12.5 kb, A2: 4.0 kb) with a constant band at 3.0 kb; TaqI (Bl: 2.9 kb, B2: 2.2 kb). The ...
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Source/Description: NB84 is a 1.0 kb Hindm-EcoRI fragment isolated from two pooled chromosome 22 specific libraries (AT-CC # 57733 and ATCC # 57714) and is subcloned into pUC9. Polymorphism: PstI digestion of genomic DNA and hybridization with the probe detects a two allele polymorphism: 4.6 kb (Al) and 4.0 kb (A2). No constant bands were present. Chromosomal Localization: Regional localization...
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Tests of 29 human variable number of tandem repeat probes in inbred mouse lines showed that 80% (23/29) cross-hybridize, and 48% (14/29) produce multiple band, minisatellite polymorphisms (fingerprint patterns). Mini-satellite-type polymorphisms detected by 11 probes were characterized in eight different strains; on average, 240 polymorphic differences were detected between pairs of strains. Re...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.4.926-a