A novel 3′ extension technique using random primers in RNA-PCR

MRPrimerV: a database of PCR primers for RNA virus detection

Many infectious diseases are caused by viral infections, and in particular by RNA viruses such as MERS, Ebola and Zika. To understand viral disease, detection and identification of these viruses are essential. Although PCR is widely used for rapid virus identification due to its low cost and high sensitivity and specificity, very few online database resources have compiled PCR primers for RNA v...

Improved quantitative PCR using nested primers.

Quantitative PCR can often be improved by conducting the amplification with nested primers. First, fewer nonspecific amplification products, which could otherwise interfere with quantitation, are produced. Often, nonspecific products can be eliminated. In these cases, relatively simple nonspecific detection techniques are suitable for quantitation. In addition, nested primer PCR provides intrin...

Characterization and Identification of F1 Hybrids in Eucalyptus by PCR Study using Random Primers

A differential pattern in the RAPD profile of Eucalyptus F1 natural hybrids FRI-4 (E. terticornis x E. camaldulensis), FRI-5 (E. camaldulensis X E. tereticornis) and controlled reciprocal crosses FRI-14 (E. torelliana X E. citriodora) along with their parental combinations was assessed through Polymerase Chain Reaction using commercially available primers. After electrophoresis of PCR product t...

PCR detection of human cytomegalovirus DNA in clinical specimens using novel UL37 exon 3 and US3 primers.

The sensitivity and specificity of novel UL37 exon 3 (UL37x3) and US3 immediate-early (IE) gene PCR primers to detect human cytomegalovirus (HCMV) DNA in clinical specimens are comparable to those of HCMV DNA polymerase (UL54) primers. The use of these IE primers increases the diagnostic performance of HCMV PCR.

Designing PCR Primers Painlessly†