A novel all-in-one strategy for purification and immobilization of β-1,3-xylanase directly from cell lysate as active and recyclable nanobiocatalyst

Abstract Background Exploring a simple and versatile technique for direct immobilization of target enzymes from cell lysate without prior purification is urgently needed. Thus, novel all-in-one strategy β-1,3-xylanase was proposed, the were covalently immobilized on silica nanoparticles via elastin-like polypeptides (ELPs)-based biomimetic silicification SpyTag/SpyCatcher spontaneous reaction. functional carriers that did not require time-consuming surface modification step quickly efficiently prepared. These could specifically immobilize SpyTag-fused pre-purification. Results The ELPs-SpyCatcher hardly leaked (0.5%), yield enzyme up to 96%. Immobilized retained 85.6% initial activity showed 88.6% recovery. Compared with free ones, improved thermal stability, elevated storage stability good pH tolerance. It also more than 70.6% after 12 reaction cycles, demonstrating its excellent reusability. Conclusions results clearly highlighted effectiveness method proposed here due improvement overall performance in respect form hydrolysis macromolecular substrates. it may have great potential conversion algae biomass as well other related fields.