A Novel Pan-FlavivirusDetection and Identification Assay Based on RT-qPCR and Microarray
نویسندگان
چکیده
منابع مشابه
A Novel Pan-Flavivirus Detection and Identification Assay Based on RT-qPCR and Microarray
The genus Flavivirus includes arthropod-borne viruses responsible for a large number of infections in humans and economically important animals. While RT-PCR protocols for specific detection of most Flavivirus species are available, there has been also a demand for a broad-range Flavivirus assay covering all members of the genus. It is particularly challenging to balance specificity at genus le...
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RealTime ready RT-qPCR Assay Development and Qualification
1 RealTime ready Assay Production and Function Testing 2 Production 2 Function Testing 2 A Census on 10,000+ RealTime ready Assays 6 Selecting the Design Sequence 6 Assay Design 6 Amplification Efficiencies 8 Bibliography References 9 M. Dietrich, U. H. Grepl, B. Kramer and H. Walch Roche Diagnostics GmbH Nonnenwald 2 82377 Penzberg / Germany RealTime ready RT-qPCR Assay Development and Qualifi...
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Human coronaviruses HCoV-OC43, HCoV-229E, HCoV-NL63 and HCoV-HKU1 are common respiratory viruses associated with acute respiratory infection. They have a global distribution. Rapid and accurate diagnosis of HCoV infection is important for the management and treatment of hospitalized patients with HCoV infection. Here, we developed a melting curve-based multiplex RT-qPCR assay for simultaneous d...
متن کاملA Facile and Specific Assay for Quantifying MicroRNA by an Optimized RT-qPCR Approach
BACKGROUND The spatiotemporal expression patterns of microRNAs (miRNAs) are important to the verification of their predicted function. RT-qPCR is the accepted technique for the quantification of miRNA expression; however, stem-loop RT-PCR and poly(T)-adapter assay, the two most frequently used methods, are not very convenient in practice and have poor specificity, respectively. RESULTS We hav...
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ژورنال
عنوان ژورنال: BioMed Research International
سال: 2017
ISSN: 2314-6133,2314-6141
DOI: 10.1155/2017/4248756