A phage P22 gene controlling integration of prophage

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A phage P22 gene controlling integration of prophage.

A mutually noncomplementing group of phage P22 mutants (L mutants) have been isolated which cannot accomplish a late step necessary for prophage integration. L mutants produce turbid plaques and lead to cell survival on high multiplicity infection, but the infecting phage genomes are incapable of integrating as prophage and are progressively diluted among the segregating cells. L mutants can be...

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Mutations of phage P22 affecting phage DNA synthesis and lysogenization.

Two temperature-sensitive mutants of phage P22, ts18.1 and ts12.1, are described which have recessive DNA-negative phenotypes. At high temperature they fail to synthesize phage DNA. In mixed infection with the two mutants or either mutant and wild-type phage, DNA synthesis and phage production are normal. In addition, under non-permissive conditions, these mutants fail to lysogenize and show do...

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Induction of P22 prophage in Salmonella typhimurium by hycanthone.

Hycanthone was found to be an inducer of P22 prophage in Salmonella typhimurium LT-2(P22).

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Recombinational circularization of Salmonella phage P22 DNA.

The development of phage P22 following infection involves a mandatory recombination step, the timing of which is the same in both the lytic and lysogenic pathways. Covalently circular molecules of phage DNA were identified by alkaline sucrose sedimentation of infected cell lysates. The time of appearance of these structures corresponds to the time of the essential recombination step. On infecti...

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Sequence of a DNA injection gene from Salmonella typhimurium phage P22.

In the Salmonella phage P22, the presence of three structural proteins in the phage particle, gp7, gpl6, and gp20 is required for successful phage DNA injection (1, 2). The genes coding for these proteins are contiguous, occupy about 4 kilobases in the late operon of P22 and are located between the imml region and genes which specify other capsid proteins. The specific function of any one of th...

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ژورنال

عنوان ژورنال: Virology

سال: 1967

ISSN: 0042-6822

DOI: 10.1016/0042-6822(67)90164-x