A qa2+-pGEM vector for Neurospora transformations
نویسندگان
چکیده
منابع مشابه
A modified unique site elimination mutagenesis in constructing a chloramphenicol resistance-encoding pGEM vector.
Recombinant DNA research requires new and versatile cloning vectors. Many of the popular plasmid vectors, such as the pUC (4), pTZ (Amersham, Arlington Heights, IL, USA) and pGEM (Promega, Madison, WI, USA) series, share common characteristics, including high copy number, sizes of around 3 kbp and the presence of a multiple cloning sequence (MCS) within the coding sequence for a part of the la...
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A highly efficient cloning vector was constructed for cloning PCR products by inserting an 80 bp DNA fragment into pGEM-5zf (+) vector. The Xcm I digestion of this vector gave rise to a 3' overhanging deoxythymidine offering the possibility of cloning PCR products with 3' adenosine overhang created by Taq DNA polymerase. Furthermore, two EcoR I sites were added to the construct for identificati...
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Background and Aims: In humans the group A rotaviruses are associated with endemic diarrhea in children under the age of 5, leading to approximately 800,000 deaths every year. Introduction of rotavirus vaccines into childhood immunization programs can contribute to substantial reduction in mortality from rotavirus gastroenteritis in developing countries and virtually eliminating hospitalization...
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1 Vector spaces A vector space is a nonempty set V , whose objects are called vectors, equipped with two operations, called addition and scalar multiplication: For any two vectors u, v in V and a scalar c, there are unique vectors u + v and cu in V such that the following properties are satisfied. 1. u + v = v + u, 2. (u + v) + w = u + (v + w), 3. There is a vector 0, called the zero vector, su...
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a highly efficient cloning vector was constructed for cloning pcr products by inserting an 80 bp dna fragment into pgem-5zf (+) vector. the xcm i digestion of this vector gave rise to a 3’ overhanging deoxythymidine offering the possibility of cloning pcr products with 3' adenosine overhang created by taq dna polymerase. furthermore, two ecor i sites were added to the construct for identificati...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1992
ISSN: 1941-4765
DOI: 10.4148/1941-4765.1437