A Rapid Method for Detecting and Quantifying Bacterial DNA in Rust Fungal DNA Samples
نویسندگان
چکیده
منابع مشابه
A rapid method for detecting and quantifying bacterial DNA in rust fungal DNA samples.
Bacterial DNA contamination of rust fungal DNA can be a significant problem for sequencing the rust fungus. Sequence assembly is much more difficult if the sequence contigs are mixed with bacterial sequence. A quantitative real-time polymerase chain reaction (qPCR) assay was developed to quantify bacterial DNA within rust fungal DNA samples and the results were compared with those obtained from...
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based on the similarity of prokaryotic dna to that of mitochondrial dna, a rapid and efficient method for dna purification from mitochondrial extracts obtained from different sources, was carried out based on the modification method described by marmur (1983) which resulted in a high degree of purity (a260/a280=2). in addition we have applied the same technique for the first time to purify the ...
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ژورنال
عنوان ژورنال: Phytopathology®
سال: 2008
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto-98-1-0115