A refined DNA methylation detection method using MspJI coupled quantitative PCR
نویسندگان
چکیده
منابع مشابه
A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI
BACKGROUND Fragmentation at random nucleotide locations is an essential process for preparation of DNA libraries to be used on massively parallel short-read DNA sequencing platforms. Although instruments for physical shearing, such as the Covaris S2 focused-ultrasonicator system, and products for enzymatic shearing, such as the Nextera technology and NEBNext dsDNA Fragmentase kit, are commercia...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2017
ISSN: 0003-2697
DOI: 10.1016/j.ab.2017.06.006