A simple and sensitive assay for ascorbate using a plate reader
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چکیده
منابع مشابه
A simple and sensitive assay for ascorbate using a plate reader.
We have developed a rapid, inexpensive, and reliable assay for the determination of ascorbate using a plate reader. In this assay, ascorbic acid is oxidized to dehydroascorbic acid using Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidinyloxy) and then reacted with o-phenylenediamine to form the condensation product, 3-(dihydroxyethyl)furo[3,4-b]quinoxaline-1-one. The rate of appearance of this pro...
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no abstract
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2007
ISSN: 0003-2697
DOI: 10.1016/j.ab.2007.03.002