A simple method for the analysis of extracellular vesicles enriched for exosomes from human serum by capillary electrophoresis with ultraviolet diode array detection

Extracellular vesicles (EVs) are membrane enclosed (<1 µm), such as exosomes (30-150 nm), involved in cell communication, which have important biological implications. In this study, EV preparations were enriched for from human serum by polyethylene glycol (PEG) precipitation. Different variables of the PEG precipitation method (i.e. concentration PEG, filtration and centrifugation resuspended pellets) evaluated measuring size isolated particles dynamic light scattering (DLS) nanoparticle tracking analysis (NTA). addition, a novel capillary electrophoresis-ultraviolet diode array (CE-UV-DAD) was developed to obtain characteristic multiwavelength electrophoretic profiles preparations. Using precipitated with 10% m/v background electrolyte (BGE) 0.1 M Tris 0.25 boric acid at pH 7.9 0.5% hydroxypropyl cellulose (HPC) allowed reducing adsorption EVs inner wall fused silica separation capillary. Sodium dodecyl sulfate (SDS) 0.1% also necessary enhance dispersibility, while homogenizing charge improve size-dependent induced HPC. Under these optimized conditions, profile preparation standard obtained, showed excellent reproducibility appropriate times. The obtained fingerprints simple, effective complementary tool quality control